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PMID: 6932042 Published · ppublish English Journal Article

Development of chemotactic responsiveness in myeloid precursor cells: studies with a human leukemia cell line.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 6 ·1980-06-00 ·Pages 3664-8

Fontana JA, Wright DG, Schiffman E, Corcoran BA, Deisseroth AB

Abstract

We have studied the events that occur during the development of chemotaxis in HL60, a promyelocytic leukemia cell line that acquires the features of mature neutrophils when exposed to dimethylformamide (DMF). Chemotactic function first appears between 48 and 96 hr of DMF induction and is associated not only with the coincidental development of deformability, spontaneous motility, greatly increased binding of fMet-Leu-Phe, and orientation but also with decreasing cell size and pleomorphism of nuclei. Surface adhesiveness develops earlier (36-48 hr) and is coincident with a 10-fold increase in protein synthesis not seen in other DMF-inducible cell lines. This burst of protein synthesis precedes the expression of chemotactic function. These studies show that the HL60 cell line can provide a useful model for delineating control mechanisms responsible for the development of complex cellular functions present in differentiated myeloid cells in humans.

MeSH Terms
Animals Cell Adhesion/drug effects Cell Differentiation/drug effects Cell Line Chemotaxis, Leukocyte/drug effects Dimethylformamide/pharmacology Hematopoietic Stem Cells Humans Leukemia, Erythroblastic, Acute/immunology Leukemia, Experimental/immunology Leukemia, Myeloid/immunology Mice N-Formylmethionine/analogs & derivatives,metabolism N-Formylmethionine Leucyl-Phenylalanine Oligopeptides/metabolism Protein Biosynthesis
Chemicals
Oligopeptides N-Formylmethionine N-Formylmethionine Leucyl-Phenylalanine Dimethylformamide
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Fontana J A
Wright D G
Schiffman E
Corcoran B A
Deisseroth A B
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.6.3664
Published
1980-06-00
Pages
3664-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349678
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.6.3664 PMC Full Text View journal details
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