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PMID: 6905765 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Stimulation of human cardiac adenylate cyclase by vanadate.

Basic research in cardiology ·Vol. 75 ·No. 3 ·1980-00-00 ·Pages 433-7

Krawietz W, Werdan K, Erdmann E

Abstract

Experiments have been carried out to characterize the influence of the positive inotropic trace element vanadium (used as Na3VO4) on beta-adrenergic receptor coupled adenylate cyclase activity from human myocardium. Na3VO4 (10(4)M) stimulates basal activity as well as isoprenaline (10 microM)- and Mg2+ (20 mM) activated enzyme activity 1.5-2.4-fold. In contrast, adenylate cyclase activity in the presence of maximally activating concentrations of Gpp(NH)p (10 microM) cannot be further increased by Na3VO4. The results confirm the assumption (5) that vanadate stimulates adenylate cyclase by interacting with the nucleotide-binding site of this enzyme.

MeSH Terms

Adenylyl Cyclases/metabolism Enzyme Activation Heart/drug effects Humans Isoproterenol/pharmacology Myocardium/enzymology Vanadates Vanadium/metabolism,pharmacology

Chemicals

Vanadium Vanadates Adenylyl Cyclases Isoproterenol

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Krawietz W

Werdan K

Erdmann E

References (12)

12 references, click to expand

Positive inotropism of vanadate in cat papillary muscle.
Nature. 1978 Sep 7;275(5675):67 PMID: 683344
The effect of epinephrine on adenosine 3', 5'-phosphate levels in the isolated perfused rat heart.
Mol Pharmacol. 1965 Sep;1(2):168-77 PMID: 5835697
Membrane adenosinetriphosphatase: a digitalis receptor?
Science. 1977 Nov 11;198(4317):569-74 PMID: 144320
Vanadate is a potent (Na,K)-ATPase inhibitor found in ATP derived from muscle.
J Biol Chem. 1977 Nov 10;252(21):7421-3 PMID: 144127
Mechanism of adenylate cyclase activation by cholera toxin: inhibition of GTP hydrolysis at the regulatory site.
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3307-11 PMID: 198781
Stimulatory effect of vanadate on the adenylate cyclase of cardiac tissue.
Biochem Pharmacol. 1979 Aug 15;28(16):2517-20 PMID: 228676
Effects of vanadate ions on action potentials and tension development in papillary muscles and cultured heart muscle cells.
Basic Res Cardiol. 1980 May-Jun;75(3):472-7 PMID: 6255936
Effects of vanadate in cultured rat heart muscle cells. Vanadate transport, intracellular binding and vanadate-induced changes in beating and in active cation flux.
Biochim Biophys Acta. 1980 Apr 10;597(2):364-83 PMID: 7370255
A highly sensitive adenylate cyclase assay.
Anal Biochem. 1974 Apr;58(2):541-8 PMID: 4827395
Role of adenine and guanine nucleotides in the activity and response of adenylate cyclase systems to hormones: evidence for multisite transition states.
Adv Cyclic Nucleotide Res. 1975;5:3-29 PMID: 236641
Activation of adenylate cyclase by vanadate.
Nature. 1979 Jan 11;277(5692):143-5 PMID: 215916
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713

Article Info

Journal

Basic research in cardiology

Abbr.

Basic Res Cardiol

ISSN

0300-8428

DOI

10.1007/BF01908407

Published

1980-00-00

Pages

433-7

Language

English

Region

Germany

NLM ID

0360342

Subset

External Links

PubMed source DOI: 10.1007/BF01908407 View journal details

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