Nucleotide sequence of Scenedesmus obliquus cytoplasmic initiator tRNA.

Nucleic acids research ·Vol. 8 ·No. 4 ·1980-02-25 ·Pages 715-29

Olins PO, Jones DS

Abstract

The cytoplasmic initiator tRNA from the green alga Scenedesmus obliquus has been purified and its sequence shown to be p A G C U G A G-U m G m G C G C A G D G G A A G C G psi m G A psi G G G C U C A U t A A--C C C A U A G m G D m C A C A G G A U C G m A A A C C U Gm U C U C A--G C U A C C A-O H. The sequence has been deduced and confirmed using several different P-post labelling techniques. The sequence is similar to those of other eukaryotic cytoplasmic initiator tRNAs and it has the sequence G A U C in place of the usual G T psi C. Although it resembles lower eukaryotic species in having a U preceding the anticodon and a modified G in the T psi C stem, in overall homology it is closer to the higher eukaryotic than to the fungal initiator tRNAs.

MeSH Terms

Base Sequence Chlorophyta/genetics Cytoplasm/metabolism Eukaryotic Cells/metabolism Fungi/genetics Methionine/metabolism Methods RNA, Transfer/genetics RNA, Transfer, Amino Acyl/genetics

Chemicals

RNA, Transfer, Amino Acyl RNA, Transfer Methionine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Olins P O

Jones D S

References (27)

27 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

Published

1980-02-25

Pages

715-29

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327305

Subset

Databases

GENBANK

K00314

External Links

PubMed source PMC Full Text View journal details

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Nucleotide sequence of Scenedesmus obliquus cytoplasmic initiator tRNA.

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