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PMID: 689707 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of hydrogen peroxide as a photoproduct toxic to human cells in tissue-culture medium irradiated with "daylight" fluorescent light.

In vitro ·Vol. 14 ·No. 8 ·1978-08-00 ·Pages 715-22

Wang RJ, Nixon BR

Abstract

Hydrogen peroxide, lethal for human cells, is produced in Dulbecco's modified Eagle's tissue culture medium when exposed to "daylight" fluorescent light. Addition of pure H2O2 and use of the enzyme catalase demonstrate that about 40% of the toxicity in irradiated medium is due to generated peroxide. Riboflavin and tryptophan, or riboflavin and tyrosine, are the components necessary for formation of lethal levels of H2O2 during light exposure.

MeSH Terms
Catalase/pharmacology Cell Line Cell Survival/drug effects Culture Media/radiation effects Hydrogen Peroxide/toxicity Light Riboflavin Tryptophan Tyrosine
Chemicals
Culture Media Tyrosine Tryptophan Hydrogen Peroxide Catalase Riboflavin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wang R J
Nixon B R
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24 references, click to expand
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Article Info
Journal
In vitro
Abbr.
In Vitro
ISSN
0073-5655
DOI
10.1007/BF02616168
Published
1978-08-00
Pages
715-22
Language
English
Region
United States
NLM ID
0063733
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02616168
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