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PMID: 687358 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Increased calcium-ion influx is a component of capacitation of spermatozoa.

The Biochemical journal ·Vol. 172 ·No. 3 ·1978-06-15 ·Pages 549-56

Singh JP, Babcock DF, Lardy HA

Abstract

Capacitation (modifications required for gamete fusion) is produced by incubating guinea-pig spermatozoa in vitro in a chemically defined medium. It is shown that during such incubation a net uptake of Ca2+ by the sperm occurs in two distinguishable phases. An initial loose association of Ca2+, possibly to surface sites, is unaffected by agents (Mg2+, inhibitors of mitochondiral function) that prevent or delay the exocytotic spermatozoal acrosome reaction. The time course of a secondary Ca2+ uptake parallels or slightly precedes the time course of the acrosome reaction. This parallelism is maintained during a variety of treatments that either expedite (local anaesthetics, ionophore A23187, Triton X-100) or delay (Mg2+, low external Ca2+) the acrosome reaction. We conclude that the secondary Ca2+ influx described herein apparently serves to link alterations of the spermatozoal membrane to subsequent contractile and secretory components of the capacitation sequence.

MeSH Terms
Acrosome/metabolism Animals Calcium/metabolism Cell Membrane Permeability Guinea Pigs In Vitro Techniques Kinetics Male Sperm Capacitation Sperm Motility/drug effects Spermatozoa/metabolism Time Factors
Chemicals
Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Singh J P
Babcock D F
Lardy H A
References (41)
41 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1720549
Published
1978-06-15
Pages
549-56
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1185730
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1720549 PMC Full Text View journal details
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