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PMID: 687357 Published · ppublish English Journal Article

Distinctive effects of glucagon on gluconeogenesis and ketogenesis in hepatocytes isolated from normal and biotin-deficient rats.

The Biochemical journal ·Vol. 172 ·No. 3 ·1978-06-15 ·Pages 517-21

Siess EA, Brocks DG, Wieland OH

Abstract

In hepatocytes from 48 h-starved rats identical glucagon dose-response curves were obtained for the stimulation of gluconeogenesis from lactate, for ketogenesis and for the decreasing of the C5-dicarboxylate pool. Glucagon (20 nM) caused a 5-fold increase in 3-hydroxybutyrate formation, but decreased acetoacetate production 50% of that of the control. In hepatocytes from biotin-deficient rats glucagon no longer stimulated gluconeogenesis from lactate, but still produced its effects on the mitochondrial redox state and the C5-dicarboxylate pool. The results suggest that the primary site of the hormone action on gluconeogenesis is located within the mitochondria rather than in the cytosol.

MeSH Terms
Animals Biotin/deficiency Dose-Response Relationship, Drug Glucagon/pharmacology Gluconeogenesis/drug effects In Vitro Techniques Ketone Bodies/biosynthesis Liver/cytology,drug effects,metabolism Male Rats
Chemicals
Ketone Bodies Biotin Glucagon
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Siess E A
Brocks D G
Wieland O H
References (33)
33 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1720517
Published
1978-06-15
Pages
517-21
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1185726
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1720517 PMC Full Text View journal details
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