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PMID: 686146 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Relation between high energy phosphate and lethal injury in myocardial ischemia in the dog.

The American journal of pathology ·Vol. 92 ·No. 1 ·1978-07-00 ·Pages 187-214

Jennings RB, Hawkins HK, Lowe JE, Hill ML, Klotman S, Reimer KA

Abstract

The relationship between progressive depletion of high energy phosphate and the onset of lethal cell injury in ischemic myocardium following coronary occlusion has been evaluated. Myocardial ischemia was induced by proximal occlusion of the circumflex coronary artery for 15, 30, 40, or 60 minutes. Cell injury in the severely ischemic posterior papillary muscle (PP) was evaluated by electron microscopy and by measuring the capacity of slices of the injured PP to maintain electrolytes, resynthesize high energy phosphate, and exclude inulin during in vitro incubation. ATP content in the ischemic myocardium decreased to 35%, 9%, 7%, and 5% of control values after 15, 30, 40, and 60 minutes of ischemia, respectively, and was associated with a corresponding depletion of total adenine nucleotides. The loss of 65% of the ATP after 15 minutes of ischemia (reversible injury) was associated with only minimal ultrastructural changes and no significant defects of electrolytes in incubated slices. However, the depletion of over 90% of the ATP after 40 minutes of ischemia (irreversible injury) was associated with significant fine structural changes and markedly altered cell volume regulation. The results suggest a close relationship between the marked depletion of high energy phsophates and the development of lethal injury in acutely ischemic myocardium.

MeSH Terms
Adenosine Triphosphate/metabolism Animals Cell Count Coronary Disease/metabolism,pathology Dogs Female Magnesium/metabolism Male Mitochondria, Heart/ultrastructure Myocardium/metabolism,ultrastructure Myofibrils/ultrastructure Potassium/metabolism Sarcolemma/ultrastructure Sodium/metabolism
Chemicals
Adenosine Triphosphate Sodium Magnesium Potassium
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Jennings R B
Hawkins H K
Lowe J E
Hill M L
Klotman S
Reimer K A
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51 references, click to expand
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1978-07-00
Pages
187-214
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC2018580
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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