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PMID: 6860315 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Coupling ratio of electrogenic Na+-alanine cotransport in isolated rat hepatocytes.

The Biochemical journal ·Vol. 210 ·No. 2 ·1983-02-15 ·Pages 621-4

Kristensen LO, Folke M

Abstract

Na+-alanine cotransport across the cell membrane in isolated rat hepatocytes was studied. Changes in the cell membrane potential associated with the transport of alanine interfere with determination of the Na+-alanine coupling ratio of the cotransport. With valinomycin present to 'clamp' the cell membrane potential, a coupling ratio of 1:1 for the Na+-alanine influx was obtained.

MeSH Terms
Alanine/metabolism Animals Biological Transport/drug effects Female In Vitro Techniques Liver/cytology,drug effects,metabolism Membrane Potentials/drug effects Rats Rats, Inbred Strains Sodium/metabolism Valinomycin/pharmacology
Chemicals
Valinomycin Sodium Alanine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kristensen L O
Folke M
References (12)
12 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2100621
Published
1983-02-15
Pages
621-4
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1154265
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2100621 PMC Full Text View journal details
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