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PMID: 6857999 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Proteins encoded near the adenovirus late messenger RNA leader segments.

Virology ·Vol. 127 ·No. 1 ·1983-05-00 ·Pages 112-23

Lewis JB, Anderson CW

Abstract

Small fragments of adenovirus 2 DNA cloned into the single-strand phage M13 were used to select adenoviral messenger RNAs transcribed from the R-strand between map positions 16 and 30. Cell-free translation of these mRNAs produced proteins of 13.5K, 13.6K, and 11.5K, respectively encoded between the first and second segments of the tripartite major late leader, within the "i"-leader segment, and immediately preceding the third leader segment. Partial sequence analysis of the 13.6K protein is consistent with the hypothesis that it is encoded within the i-leader segment.

MeSH Terms
Adenoviruses, Human/genetics Base Sequence Cloning, Molecular Coliphages/genetics DNA, Single-Stranded/analysis DNA, Viral/analysis Peptides/analysis RNA, Messenger/analysis RNA, Viral/analysis Viral Proteins/analysis
Chemicals
DNA, Single-Stranded DNA, Viral Peptides RNA, Messenger RNA, Viral Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lewis J B
Anderson C W
Article Info
Journal
Virology
Abbr.
Virology
ISSN
0042-6822
DOI
10.1016/0042-6822(83)90376-8
Published
1983-05-00
Pages
112-23
Language
English
Region
United States
NLM ID
0110674
Subset
IM
Grants
NCI NIH HHS · CA15704 · United States
Databases
GENBANK
J01917, J01918, J01919, J01920, J01921, J01922, J01923, J01924, J01925, J01926, J01927, J01928, J01929, J01930, J01931, J01932, J01933, J01934, J01935, J01936, J01937, J01938, J01939, J01940, J01941, J01942, J01943, J01944, J01945, J01946
External Links
PubMed source DOI: 10.1016/0042-6822(83)90376-8 View journal details
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