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PMID: 6853447 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Control of catechol meta-cleavage pathway in Alcaligenes eutrophus.

Journal of bacteriology ·Vol. 154 ·No. 3 ·1983-06-00 ·Pages 1363-70

Hughes EJ, Bayly RC

Abstract

Alcaligenes eutrophus 335 (ATCC 17697) metabolizes phenol and p-cresol via a catechol meta-cleavage pathway. Studies with mutant strains, each defective in an enzyme of the pathway, showed that the six enzymes assayed are induced by the primary substrate. Studies with a putative polarity mutant defective in the expression of aldehyde dehydrogenase suggested that the structural genes encoding this and subsequent enzymes of the pathway exist in the same operon. From studies with mutant strains that constitutively synthesize catechol 2,3-oxygenase and subsequent enzymes and from the coordination of repression of these enzymes by p-toluate, benzoate, and acetate, it is proposed the catechol 2,3-oxygenase structural gene is situated in this operon (2,3-oxygenase operon). Studies with regulatory mutant strains suggest that the 2,3-oxygenase operon is under negative control.

MeSH Terms
Alcaligenes/enzymology,genetics Benzoates/pharmacology Benzoic Acid Catechol 2,3-Dioxygenase Catechols/metabolism Cresols/metabolism Dioxygenases Enzyme Induction Enzyme Repression Gene Expression Genes, Regulator Lactates/metabolism Lactic Acid Mixed Function Oxygenases/metabolism Mutation Operon Oxygenases/genetics,metabolism Phenol Phenols/metabolism
Chemicals
Benzoates Catechols Cresols Lactates Phenols 4-cresol Phenol Lactic Acid Benzoic Acid 4-toluic acid Mixed Function Oxygenases Oxygenases Dioxygenases Catechol 2,3-Dioxygenase phenol 2-monooxygenase catechol
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hughes E J
Bayly R C
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25 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.154.3.1363-1370.1983
Published
1983-06-00
Pages
1363-70
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217612
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.154.3.1363-1370.1983 PMC Full Text View journal details
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