Measurement of the translational diffusion constant of G-actin by photon correlation spectroscopy.

Journal of muscle research and cell motility ·Vol. 4 ·No. 1 ·1983-02-00 ·Pages 95-101

Montague C, Rhee KW, Carlson FD

Abstract

Photon correlation spectroscopic measurements on monomeric actin have yielded a translational diffusion constant of 8.13 X 10(-7) cm2 s-1 after correction for the contribution of the back-reflections of the main beam. This value corresponds to a sphere with a radius of 2.6 nm and 50% hydration, or to a prolate ellipsoid with axes 2.0 and 4.0 nm and 30% hydration.

MeSH Terms

Actins/metabolism Animals Diffusion Muscles/metabolism Protein Conformation Rabbits Spectrum Analysis Viscosity

Chemicals

Actins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Montague C

Rhee K W

Carlson F D

References (10)

10 references, click to expand

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Three-dimensional structure of the complex of skeletal muscle actin and bovine pancreatic DNAse I at 6-A resolution.
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Dynamic light-scattering evidence for the flexibility of native muscle thin filaments.
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FEBS Lett. 1975 Mar 15;52(1):8-12 PMID: 1168157
Structure of crystalline actin sheets.
Nature. 1980 Nov 20;288(5788):296-8 PMID: 6893623
Detection of conformational changes in actin by proteolytic digestion: evidence for a new monomeric species.
J Mol Biol. 1976 Jul 15;104(4):777-92 PMID: 957440
MOLECULAR CHARACTERISTICS OF G-ADP ACTIN.
Arch Biochem Biophys. 1964 Sep;107:441-8 PMID: 14234493
Identification of a factor in conventional muscle actin preparations which inhibits actin filament self-association.
Biochem Biophys Res Commun. 1980 Sep 16;96(1):18-27 PMID: 6893667
The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.
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Article Info

Journal

Journal of muscle research and cell motility

Abbr.

J Muscle Res Cell Motil

ISSN

0142-4319

DOI

10.1007/BF00711960

Published

1983-02-00

Pages

95-101

Language

English

Region

Netherlands

NLM ID

8006298

Subset

External Links

PubMed source DOI: 10.1007/BF00711960 View journal details

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Measurement of the translational diffusion constant of G-actin by photon correlation spectroscopy.

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