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PMID: 6816951 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structure of the lipid-containing bacteriophage PRD1: disruption of wild-type and nonsense mutant phage particles with guanidine hydrochloride.

Journal of virology ·Vol. 44 ·No. 3 ·1982-12-00 ·Pages 1031-8

Bamford D, Mindich L

Abstract

The lipid-containing bacteriophage PRD1 was disrupted, and the subviral particles were studied. Guanidine treatment released two phage proteins (P3 and P5). These proteins form the polyhedral capsid. The remaining phage proteins were associated with the phage membrane vesicle. The vesicle was capable of forming a tubular structure. The isolated phage membrane vesicles aggregated readily. We found that aggregation and tube formation were associated with specific phage proteins (P11 and P18, respectively) by using protease treatment and an analysis of nonsense mutant phage particles. In addition, the possibility that free vesicles might be precursors to empty virions was studied.

MeSH Terms

Bacteriophages/genetics Cell Membrane/ultrastructure Guanidine Guanidines/pharmacology Lipids/analysis Microscopy, Electron Mutation Pseudomonas aeruginosa/genetics Salmonella Phages/genetics Salmonella typhimurium/genetics Viral Proteins/genetics

Chemicals

Guanidines Lipids Viral Proteins Guanidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Bamford D

Mindich L

References (15)

15 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.44.3.1031-1038.1982

Published

1982-12-00

Pages

1031-8

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC256363

Subset

Grants

NIAID NIH HHS · AI-09861 · United States

External Links

PubMed source DOI: 10.1128/JVI.44.3.1031-1038.1982 PMC Full Text View journal details

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Structure of the lipid-containing bacteriophage PRD1: disruption of wild-type and nonsense mutant phage particles with guanidine hydrochloride.

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