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PMID: 6811440 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Lymphokine-induced mycobacteriostatic activity in mouse pleural macrophages.

Infection and immunity ·Vol. 37 ·No. 2 ·1982-08-00 ·Pages 786-93

Zlotnik A, Crowle AJ

Abstract

We have developed an in vitro assay to study the intramacrophage replication of Mycobacterium tuberculosis or Mycobacterium bovis BCG. In this assay, extracellular mycobacterial replication was avoided by using a small infecting inoculum and a new method for efficiently removing nonphagocytocytized bacilli. The ability of different mycobacterial strains to replicate in mouse pleural macrophages correlated with their virulence. The effect of lymphokines prepared by incubating spleen cells from BCG-immunized mice with mycobacterial antigens was tested with this assay. In the presence of lymphokines, mouse pleural macrophages were able to inhibit the intramacrophage replication of both BCG and M. tuberculosis. This assay is a useful in vitro model for studying antibacterial cellular immunity expressed by macrophages.

MeSH Terms
Animals Cells, Cultured Female Growth Inhibitors/pharmacology Lung/cytology Lymphokines/pharmacology Macrophage Activation Macrophages/microbiology,ultrastructure Mice Mice, Inbred BALB C Mycobacterium/growth & development,ultrastructure Mycobacterium bovis/growth & development Mycobacterium tuberculosis/growth & development Phagocytosis
Chemicals
Growth Inhibitors Lymphokines
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zlotnik A
Crowle A J
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18 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.37.2.786-793.1982
Published
1982-08-00
Pages
786-93
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC347598
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.37.2.786-793.1982 PMC Full Text View journal details
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