Home LiteratureArticle Details
PMID: 6801025 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

DNA isolated from Mycobacterium leprae: genome size, base ratio, and homology with other related bacteria as determined by optical DNA-DNA reassociation.

Journal of bacteriology ·Vol. 150 ·No. 1 ·1982-04-00 ·Pages 414-7

Imaeda T, Kirchheimer WF, Barksdale L

Abstract

DNA derived from Mycobacterium leprae (grown in armadillos) was isolated, purified, and analyzed spectrophotometrically. The genome size and the guanine-plus-cytosine content of M. leprae were 1.3 x 10(9) and 55.8%, respectively. Among selected strains of mycobacterial, nocardial, and corynebacterial species, Corynebacterium sp. 2628 LB, isolated from a human leprosy patient, showed the highest DNA homology with M. leprae. Of the DNAs derived from mycobacteria, those of M. tuberculosis and M. scrofulaceum showed a comparatively high reassociation with the DNMA of M. liprae.

MeSH Terms
Base Composition Base Sequence Corynebacterium/genetics Cytosine/analysis DNA, Bacterial/analysis Guanine/analysis Mycobacterium/genetics Mycobacterium leprae/genetics Mycobacterium tuberculosis/genetics Nucleic Acid Renaturation
Chemicals
DNA, Bacterial Guanine Cytosine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Imaeda T
Kirchheimer W F
Barksdale L
References (30)
30 references, click to expand
  1. Electron microscopy. Approach to leprosy research.
    Int J Lepr. 1965 Jul-Sep;33(3):Suppl:669-88 PMID: 5322710
  2. Media and methods for phage-typing mycobacteria.
    Bull World Health Organ. 1966;35(4):563-8 PMID: 5297555
  3. Chemical analyses of the cell wall of the murine leprosy bacillus.
    J Bacteriol. 1968 Sep;96(3):860-1 PMID: 5732516
  4. Ultrastructural changes in bacteria isolated from cases of leprosy.
    J Bacteriol. 1969 Nov;100(2):1062-75 PMID: 5359609
  5. Correlation of melting temperature and cesium chloride buoyant density of bacterial deoxyribonucleic acid.
    J Bacteriol. 1970 Feb;101(2):333-8 PMID: 5413818
  6. Method for isolation of deoxyribonucleic acid from mycobacteria.
    J Bacteriol. 1970 Nov;104(2):1020-1 PMID: 5489429
  7. Nucleic acid homology in the genus Mycobacterium.
    J Bacteriol. 1970 Nov;104(2):630-4 PMID: 4992365
  8. Quantitative aspects of deoxyribonucleic acid renaturation: base composition, state of chromosome replication, and polynucleotide homologies.
    J Bacteriol. 1971 May;106(2):608-14 PMID: 4929869
  9. Gene linkage by RNA-DNA hybridization. II. Arrangement of the redundant gene sequences for 28 s and 18 s ribosomal RNA.
    J Mol Biol. 1968 Jun 28;34(3):681-97 PMID: 5760468
  10. Immunologic analysis of Mycobacterium leprae antigens by means of diffusion-in-gel methods.
    Int J Lepr Other Mycobact Dis. 1971 Apr-Jun;39(2):105-12 PMID: 5003224
  11. Immunologic researches in leprosy. Diagnostic applications.
    Int J Lepr Other Mycobact Dis. 1971 Apr-Jun;39(2):565-73 PMID: 4948090
  12. Attempts to establish the armadillo (Dasypus novemcinctus Linn.) as a model for the study of leprosy. I. Report of lepromatoid leprosy in an experimentally infected armadillo.
    Int J Lepr Other Mycobact Dis. 1971 Jul-Sep;39(3):693-702 PMID: 4948218
  13. Immunochemical identification of a defined antigen of Mycobacterium leprae.
    Infect Immun. 1972 Feb;5(2):258-9 PMID: 4117882
  14. Purification of mycobacterial deoxyribonucleic acid.
    J Bacteriol. 1972 Dec;112(3):1033-9 PMID: 4629650
  15. Relationships among mycobacteria and nocardiae based upon deoxyribonucleic acid reassociation.
    J Bacteriol. 1973 Feb;113(2):645-51 PMID: 4690961
  16. Chemical characterization of organisms isolated from leprosy patients.
    J Bacteriol. 1974 Mar;117(3):1320-9 PMID: 4813897
  17. Mycolic acids from "noncultivable" mycobacteria.
    Infect Immun. 1974 Jul;10(1):236-9 PMID: 4601900
  18. Studies of mycobacterial antigens, with special reference to Mycobacterium leprae.
    Infect Immun. 1976 Apr;13(4):1132-8 PMID: 58837
  19. Isolation of covalently closed circular DNA of high molecular weight from bacteria.
    Anal Biochem. 1976 Dec;76(2):431-41 PMID: 11708
  20. Common antigen of Mycobacterium leprae, M. lepraemurium, M. avium, and M. fortuitum in comparative studies using two different types of antisera.
    Infect Immun. 1977 May;16(2):542-6 PMID: 68004
  21. Evaluation of the immune response in mice infected with Mycobacterium marinum.
    Int J Lepr Other Mycobact Dis. 1977 Jul-Sep;45(3):228-34 PMID: 336554
  22. Antibody response in rabbits to immunization with Mycobacterium leprae.
    Infect Immun. 1977 Dec;18(3):792-805 PMID: 338491
  23. Deoxyribonucleic acid hybridization between different species of mycobacteria.
    Acta Pathol Microbiol Scand B. 1978 Apr;86(2):71-6 PMID: 696325
  24. Adsorption of mycobacteriophage D29 on Mycobacterium leprae.
    Ann Microbiol (Paris). 1978 May-Jun;129(4):563-6 PMID: 358889
  25. Immunological relatedness of ribosomes from mycobacteria, nocardiae and corynebacteria, and microorganisms in leprosy lesions.
    Infect Immun. 1978 Nov;22(2):540-7 PMID: 730371
  26. Comparison of various preparations of Mycobacterium leprae and other mycobacteria by lymphocyte stimulation.
    Int J Lepr Other Mycobact Dis. 1978 Jul-Dec;46(3-4):386-93 PMID: 83309
  27. Delayed hypersensitivity responses in mice and guinea pigs to Mycobacterium leprae, Mycobacterium vaccae, and Mycobacterium nonchromogenicum cytoplasmic proteins.
    Infect Immun. 1979 Jul;25(1):229-36 PMID: 383613
  28. Comparative studies of antigen 21 in Mycobacterium and Nocardia species: possible taxonomic relationships with Mycobacterium leprae.
    Infect Immun. 1980 Nov;30(2):413-20 PMID: 7002798
  29. Antibodies to mycobacteria in human tuberculosis. II. Response to nine defined mycobacterial antigens with evidence for an antibody common to tuberculosis and lepromatous leprosy.
    J Infect Dis. 1980 Dec;142(6):835-43 PMID: 6780633
  30. [Lipidic constituents of "Mycobacterium leprae" isolated from experimentally infected armadillo (author's transl)].
    Ann Microbiol (Paris). 1981 Jan-Feb;132A(1):19-30 PMID: 7020522
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.150.1.414-417.1982
Published
1982-04-00
Pages
414-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC220132
Subset
IM
Grants
NIAID NIH HHS · AI-60001 · United States
External Links
PubMed source DOI: 10.1128/jb.150.1.414-417.1982 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2