Difluoromethylornithine irreversibly inactivates ornithine decarboxylase of Pseudomonas aeruginosa, but does not inhibit the enzymes of Escherichia coli.

The Biochemical journal ·Vol. 200 ·No. 1 ·1981-10-15 ·Pages 69-75

Kallio A, McCann PP

Abstract

DL-alpha-Difluoromethylornithine, an enzyme-activated irreversible inhibitor of eukaryotic ornithine decarboxylase and consequently of putrescine biosynthesis, inhibited ornithine decarboxylase in enzyme extracts from Pseudomonas aeruginosa in a time-dependent manner t1/2 1 min, and also effectively blocked the enzyme activity in situ in the cell. Difluoromethylornithine, however, had no effect on the activity of ornithine decarboxylase assayed in enzyme extracts from either Escherichia coli or Klebsiella pneumoniae. However, the presence of the inhibitor in cell cultures did partially lower ornithine decarboxylase activity intracellularly in E. coli. Any decrease in the intracellular ornithine decarboxylase activity observed in E. coli and Pseudomonas was accompanied by a concomitant increase in arginine decarboxylase activity, arguing for a co-ordinated control of putrescine biosynthesis in these cells.

MeSH Terms

Carboxy-Lyases/antagonists & inhibitors,metabolism Eflornithine Escherichia coli/drug effects,enzymology Kinetics Klebsiella pneumoniae/drug effects,enzymology Ornithine/analogs & derivatives,pharmacology Ornithine Decarboxylase Inhibitors Pseudomonas aeruginosa/drug effects,enzymology

Chemicals

Ornithine Decarboxylase Inhibitors Ornithine Carboxy-Lyases arginine decarboxylase Eflornithine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kallio A

McCann P P

References (27)

27 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2000069

Published

1981-10-15

Pages

69-75

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1163503

Subset

External Links

PubMed source DOI: 10.1042/bj2000069 PMC Full Text View journal details

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Difluoromethylornithine irreversibly inactivates ornithine decarboxylase of Pseudomonas aeruginosa, but does not inhibit the enzymes of Escherichia coli.

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