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PMID: 6790568 Published · ppublish English Journal Article

Ethylenediaminetetraacetic acid (disodium salt)-labile bovine immunoglobulin M Fc binding to Brucella abortus: a cause of nonspecific agglutination.

Journal of clinical microbiology ·Vol. 14 ·No. 1 ·1981-07-00 ·Pages 32-8

Nielsen K, Stilwell K, Stemshorn B, Duncan R

Abstract

It was demonstrated by a radioimmunoassay procedure that Brucella abortus agglutinins from noninfected cattle sera, absorbed to B. abortus antigen and eluted with ethylenediaminetetraacetic acid (EDTA), was immunoglobulin M that bound to that bacterium by its Fc portion. The EDTA-eluted immunoglobulin M agglutinated intact B. abortus cells but not erythrocytes treated with B. abortus lipopolysaccharide. The specificity of the EDTA-eluted immunoglobulin was for B. abortus, although a small titer to Yersinia enterocolitica serotype O:9 was observed. In contrast, immunoglobulin M purified from the serum of a cow injected 7 days previously with heat-killed B. abortus bound to the antigen by its Fab portion, was not labile to EDTA treatment, cross-reacted extensively with Y. enterocolitica serotype O:9, and agglutinated various other bacterial antigens and normal erythrocytes.

MeSH Terms
Animals Antigen-Antibody Reactions Brucella abortus/immunology Brucellosis, Bovine/diagnosis Cattle Edetic Acid/pharmacology Female Hemagglutination Tests/veterinary Immunoglobulin Fc Fragments/immunology Immunoglobulin M/immunology Papain/pharmacology
Chemicals
Immunoglobulin Fc Fragments Immunoglobulin M Edetic Acid Papain
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nielsen K
Stilwell K
Stemshorn B
Duncan R
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32 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.14.1.32-38.1981
Published
1981-07-00
Pages
32-8
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC271898
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.14.1.32-38.1981 PMC Full Text View journal details
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