Home LiteratureArticle Details

PMID: 6788382 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The association of rhodamine - labelled alpha-actinin with actin bundles in demembranated cells.

Cell biology international reports ·Vol. 5 ·No. 6 ·1981-06-00 ·Pages 627-34

Geiger B

Abstract

Rhodamine-labelled alpha-actinin was specifically bound to actin containing filament bundles of demembranated fibroblasts, and was particularly associated with their termini. Optimal binding of rhodamine-alpha-actinin occurred at pH 6.0 - 6.2 and could be abolished by the addition of unlabeled alpha-actinin or myosin subfragment 1. The spatial relationships between the decorating alpha-actinin and several actin associated proteins was determined by double fluorescence microscopy.

MeSH Terms

Actinin/metabolism Actins/analysis Animals Cell Line Cells, Cultured Chick Embryo Contractile Proteins/analysis Cytoskeleton/metabolism Dogs Fibroblasts Filamins Hydrogen-Ion Concentration Microfilament Proteins Muscle Proteins/analysis,metabolism Rhodamines Tropomyosin/analysis Vinculin

Chemicals

Actins Contractile Proteins Filamins Microfilament Proteins Muscle Proteins Rhodamines Tropomyosin Actinin Vinculin

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Geiger B

Article Info

Journal

Cell biology international reports

Abbr.

Cell Biol Int Rep

ISSN

0309-1651

DOI

10.1016/s0309-1651(81)80015-x

Published

1981-06-00

Pages

627-34

Language

English

Region

England

NLM ID

7708050

Subset

External Links

PubMed source DOI: 10.1016/s0309-1651(81)80015-x

Analysis Services

The association of rhodamine - labelled alpha-actinin with actin bundles in demembranated cells.

Contact

WeChat Official Account