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PMID: 6787078 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Use of New York City medium for improved recovery of Neisseria gonorrhoeae from clinical specimens.

Journal of clinical microbiology ·Vol. 13 ·No. 5 ·1981-05-00 ·Pages 963-8

Granato PA, Schneible-Smith C, Weiner LB

Abstract

New York City (NYC) and Martin-Lewis (ML) media were evaluated comparatively for their ability to support the growth of Neisseria gonorrhoeae from clinical specimens. A total of 1,010 urethral, cervical, pharyngeal, and rectal specimens were collected from walk-in patients attending a clinic for sexually transmitted diseases. A total of 187 and 165 isolates of gonococci were cultivated on NYC and ML media, respectively, with 161 of these isolates being recovered on both media. Overall, the use of NYC medium resulted in a 13.3% increased recovery rate of gonococci. When gonococci were recovered on both media from primary isolation, the NYC medium supported a more luxuriant growth and a greater number of colonies, which usually resulted in the detection of positive cultures 1 day sooner than on ML medium. Both media were comparable in their ability to suppress the growth of saprophytic microorganisms. The results of this study demonstrated that the use of NYC medium markedly enhanced the recovery of N. gonorrhoeae from clinical specimens as compared to ML medium.

MeSH Terms
Cervix Uteri/microbiology Culture Media Drug Resistance, Microbial Female Gonorrhea/microbiology Humans Male Neisseria gonorrhoeae/drug effects,growth & development,isolation & purification Pharynx/microbiology Rectum/microbiology Urethra/microbiology Vancomycin/pharmacology
Chemicals
Culture Media Vancomycin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Granato P A
Schneible-Smith C
Weiner L B
References (25)
25 references, click to expand
  1. Improved medium selective for cultivation of N. gonorrhoeae and N. meningitidis.
    Public Health Rep. 1966 Jun;81(6):559-62 PMID: 4957043
  2. A SELECTIVE MEDIUM FOR THE CULTIVATION OF N. GONORRHOEAE AND N. MENINGITIDIS.
    Public Health Rep. 1964 Jan;79:49-57 PMID: 14105729
  3. Use of trimethoprim to prevent overgrowth by Proteus in the cultivation of N. gonorrhoeae.
    Br J Vener Dis. 1970 Jun;46(3):201-2 PMID: 4988099
  4. Trimethoprim as an additional selective agent in media for the isolation of N. gonorrhoeae.
    J Clin Pathol. 1970 Sep;23(6):481-3 PMID: 4990999
  5. Transgrow, a medium for transport and growth of Neisseria gonorrhoeae and Neisseria meningitidis.
    HSMHA Health Rep. 1971 Jan;86(1):30-3 PMID: 5543005
  6. Trimethoprim for the prevention of overgrowth by swarming Proteus in the cultivation of gonococci.
    Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(4):545-8 PMID: 5000798
  7. VCN--inhibited strains of Neisseria gonorrhoeae.
    HSMHA Health Rep. 1971 Nov;86(11):990-2 PMID: 5002991
  8. Comparison of a selective and a non-selective medium in the diagnosis of gonorrhoea to ascertain the sensitivity of Neisseria gonorrhoeae to vancomycin.
    Br J Vener Dis. 1972 Oct;48(5):363-8 PMID: 4345591
  9. A new medium for the isolation of pathogenic Neisseria (NYC medium). I. Formulation and comparisons with standard media.
    Health Lab Sci. 1973 Apr;10(2):44-54 PMID: 4633802
  10. A new medium for the isolation of pathogenic Neisseria (NYC medium). II. Effect of amphotericin B and trimethoprim lactate on selectivity.
    Health Lab Sci. 1973 Apr;10(2):55-60 PMID: 4633803
  11. A new medium for the isolation of pathogenic Neisseria (NYC nmedium). 3. Performance as a culture and transport medium without addition of ambient carbon dioxide.
    Health Lab Sci. 1973 Apr;10(2):61-74 PMID: 4633804
  12. Vancomycin-sensitive strains of Neisseria gonorrhoeae. A problem for the diagnostic laboratory.
    Br J Vener Dis. 1973 Oct;49(5):452-3 PMID: 4201245
  13. New system for cultivation of Neisseria gonorrhoeae.
    Appl Microbiol. 1974 Apr;27(4):802-5 PMID: 4207764
  14. NYC medium for simultaneous isolation of Neisseria gonorrhoeae, large-colony mycoplasmas, and T-mycoplasmas.
    Appl Microbiol. 1974 Jun;27(6):1041-5 PMID: 4208637
  15. Gonogrow, an improved, selective medium for the isolation of Neisseria gonorrhoeae.
    Health Lab Sci. 1974 Jul;11(3):173-7 PMID: 4209767
  16. Morphologic observations of mycoplasmas and Neisseria gonorrhoeae in associated growth patterns.
    Am J Clin Pathol. 1975 Jan;63(1):106-16 PMID: 46128
  17. Superiority of amphotericin over nystatin in Thayer-Martin medium.
    Antimicrob Agents Chemother. 1975 May;7(5):658-60 PMID: 807158
  18. Lincomycin in selective medium for the isolation of Neisseria gonorrhoeae.
    Acta Pathol Microbiol Scand B. 1975 Jun;83(3):301-4 PMID: 808098
  19. Improved transport system for Neisseria gonorrhoeae in clinical specimens.
    J Clin Microbiol. 1975 Dec;2(6):498-503 PMID: 812885
  20. Field evaluation of New York City medium in the biological environment-CO2 chamber in recovery of Neisseria gonorrhoeae and urogenital mycoplasmas.
    J Clin Microbiol. 1977 Feb;5(2):137-41 PMID: 403197
  21. Cultural diagnosis of gonorrhoea with modified New York City (MNYC) medium.
    Br J Vener Dis. 1978 Feb;54(1):36-40 PMID: 416875
  22. The selectivity of vancomycin and lincomycin in NYC medium for the recovery of N. gonorrhoeae from clinical specimens.
    Health Lab Sci. 1978 Jan;15(1):22-7 PMID: 417048
  23. An evaluation of New York City transport medium for the detection of Neisseria gonorrhoeae in clinical specimens.
    Health Lab Sci. 1975 Apr;12(2):69-75 PMID: 829908
  24. A study on the growth requirements of Neisseria gonorrhoeae and its clinical application.
    Yale J Biol Med. 1957 Jun;29(6):613-21 PMID: 13443365
  25. Recent developments in the laboratory diagnosis of gonococcal infections.
    Bull World Health Organ. 1969;40(2):245-55 PMID: 4979391
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.13.5.963-968.1981
Published
1981-05-00
Pages
963-8
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC273924
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.13.5.963-968.1981 PMC Full Text View journal details
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