Home LiteratureArticle Details
PMID: 6784122 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Molecular limits on the size of a genetic locus in Drosophila melanogaster.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 12 ·1980-12-00 ·Pages 7367-71

McGinnis W, Farrell J, Beckendorf SK

Abstract

This report places outer limits on the size of the DNA region required for expression of a Drosophila gene. This region, termed the unit of expression, includes not only the structural gene but also any cis-acting sequences that modulate its activity. The locus we have chosen, Sgs-4, codes for one of the glue proteins secreted by larval Drosophila salivary glands. Cytological deficiencies have been identified that eliminate sequences on one side or the other of Sgs-4 without affecting its expression. The ends of these deficiencies have been localized accurately with respect to restriction endonuclease sites in and near the locus. These endpoints limit the Sgs-4 structural gene and essential flanking sequences to a 16- to 19-kilobase region of the X chromosome. The results also show that there is no DNA sequence rearrangement in the Sgs-4 region during development of either the polytene larval salivary glands or adult flies.

MeSH Terms
Animals Chromosome Deletion Chromosome Mapping Drosophila melanogaster/genetics Female Gene Expression Regulation Genes Larva Molecular Weight Saliva X Chromosome
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McGinnis W
Farrell J
Beckendorf S K
References (17)
17 references, click to expand
  1. Nonessential Sequences, Genes, and the Polytene Chromosome Bands of DROSOPHILA MELANOGASTER.
    Genetics. 1978 Apr;88(4):723-42 PMID: 17248815
  2. The consequences of nullosomy for a chromosomal region affecting cyclic AMP phosphodiesterase activity in Drosophila.
    Genetics. 1977 Apr;85(4):623-8 PMID: 193764
  3. gamma-beta-Thalassaemia studies showing that deletion of the gamma- and delta-genes influences beta-globin gene expression in man.
    Nature. 1980 Feb 14;283(5748):637-42 PMID: 6153459
  4. Organization of the rosy locus in Drosophila melanogaster: further evidence in support of a cis-acting control element adjacent to the xanthine dehydrogenase structural element.
    Genetics. 1979 Feb;91(2):275-93 PMID: 109351
  5. The two-dimensional fractionation of drosophila DNA.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 2:1023-31 PMID: 98259
  6. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels.
    Biochemistry. 1968 Feb;7(2):668-74 PMID: 4868544
  7. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  8. Nucleotide sequence of the rightward operator of phage lambda.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 PMID: 1055375
  9. Position-effect variegation.
    Adv Genet. 1968;14:133-69 PMID: 4884780
  10. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  11. Characterisation of deletions which affect the expression of fetal globin genes in man.
    Nature. 1979 Jun 14;279(5714):598-603 PMID: 450109
  12. Molecular analysis of a gene in a developmentally regulated puff of Drosophila melanogaster.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7362-6 PMID: 6784121
  13. Differentiation in the salivary glands of Drosophila melanogaster: characterization of the glue proteins and their developmental appearance.
    Cell. 1976 Nov;9(3):365-73 PMID: 825230
  14. Direct correlation between a chromosome puff and the synthesis of a larval saliva protein in Drosophila melanogaster.
    Chromosoma. 1977 Jul 5;62(2):155-74 PMID: 407059
  15. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  16. The relative mutabilities of DNA in regions of the X chromosome of Drosophila melanogaster.
    Genetics. 1965 Sep;52(3):665-81 PMID: 5863912
  17. A cytogenetic analysis of cyclic nucleotide phosphodiesterase activities in Drosophila.
    Genetics. 1977 Apr;85(4):609-22 PMID: 193763
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.12.7367
Published
1980-12-00
Pages
7367-71
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC350504
Subset
IM
Grants
NICHD NIH HHS · HD 12866 · United States
External Links
PubMed source DOI: 10.1073/pnas.77.12.7367 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2