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PMID: 6784120 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Specific disruption of vimentin filament organization in monkey kidney CV-1 cells by diphtheria toxin, exotoxin A, and cycloheximide.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 12 ·1980-12-00 ·Pages 7267-71

Sharpe AH, Chen LB, Murphy JR, Fields BN

Abstract

We have examined the effect of diphtheria toxin, Pseudomonas aeruginosa exotoxin A, and cycloheximide on the CV-1 cell cytoskeleton. Within a few hours after producing an inhibition of cellular protein synthesis, all these agents specifically disrupted the organization of the vimentin filament system with no discernable effect on microtubules or microfilaments during the period of observation. Furthermore, just as the inhibition of protein synthesis by cycloheximide is reversible, so was the disruption of vimentin filaments by cycloheximide.

MeSH Terms
Animals Bacterial Toxins/pharmacology Cell Line Chlorocebus aethiops Cycloheximide/pharmacology Cytoskeleton/drug effects Diphtheria Toxin/pharmacology Exotoxins/pharmacology Muscle Proteins/pharmacology Pseudomonas aeruginosa Vimentin
Chemicals
Bacterial Toxins Diphtheria Toxin Exotoxins Muscle Proteins Vimentin Cycloheximide
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sharpe A H
Chen L B
Murphy J R
Fields B N
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21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.12.7267
Published
1980-12-00
Pages
7267-71
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC350483
Subset
IM
Grants
NIGMS NIH HHS · 5-T32 GMO 7196 · United States
NIAID NIH HHS · AI 13178 · United States
NIAID NIH HHS · K04 AI-00146 · United States
External Links
PubMed source DOI: 10.1073/pnas.77.12.7267 PMC Full Text View journal details
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