Tetracycline resistance genes from Bacillus plasmid pAB124 confer decreased accumulation of the antibiotic in Bacillus subtilis but not in Escherichia coli.
Expression of tetracycline resistance by genes originating in the Bacillus plasmid pAB124 was examined in both Bacillus subtilis and Escherichia coli host cells. Expression of resistance in B. subtilis by genes from pAB124 was inducible and associated with decreased accumulation of the antibiotic. A fragment of pAB124 carrying the genes coding for tetracycline resistance was cloned into the E. coli plasmid RSF2124. The cloned fragment conferred a low level of resistance in E. coli, but this was not associated with decreased uptake of tetracycline and was not inducible.
16 references, click to expand
-
REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.J Bacteriol. 1961 May;81(5):741-6 PMID: 16561900
-
Heterogeneity of tetracycline resistance determinants.Plasmid. 1980 Mar;3(2):99-108 PMID: 6927764
-
The generation of a ColE1-Apr cloning vehicle which allows detection of inserted DNA.Mol Gen Genet. 1975 Dec 30;142(3):239-49 PMID: 1045010
-
A freeze-squeeze method for recovering long DNA from agarose gels.Anal Biochem. 1975 May 26;66(1):213-20 PMID: 1096670
-
Bacterial resistance to the tetracyclines.Microbiol Rev. 1978 Dec;42(4):707-24 PMID: 739968
-
Characterization of Bacillus stearothermophilus plasmid pab124 and construction of deletion variants.J Gen Microbiol. 1980 Jul;119(1):109-15 PMID: 6251158
-
Evidence for more than one mechanism of plasmid-determined tetracycline resistance in Escherichia coli.J Gen Microbiol. 1980 Nov;121(1):221-9 PMID: 7019387
-
Isolation and partial characterization of four plasmids from antibiotic-resistant thermophilic bacilli.J Gen Microbiol. 1979 Oct;114(2):401-8 PMID: 232126
-
Recovery of DNA segments from agarose gels.Anal Biochem. 1980 Jan 15;101(2):339-41 PMID: 6244756
-
Chromosome replication and the division cycle of Escherichia coli B/r.J Mol Biol. 1968 Feb 14;31(3):519-40 PMID: 4866337
-
Diffusion of tetracycline across the outer membrane of Escherichia coli K-12: involvement of protein Ia.Biochem Biophys Res Commun. 1978 Jul 28;83(2):550-7 PMID: 358984
-
Plasmid-mediated tetracycline resistance in Escherichia coli involves increased efflux of the antibiotic.Biochem Biophys Res Commun. 1980 Mar 13;93(1):74-81 PMID: 6990931
-
Plasmid replication in DNA Ts mutants of Bacillus subtilis.Plasmid. 1978 Jun;1(3):405-16 PMID: 107538
-
Circularity of the Bacillus subtilis chromosome and further studies on its bidirectional replication.J Mol Biol. 1973 Jul 15;77(4):569-75 PMID: 4198889
-
Mapping of functions in the R-plasmid R388 by examination of deletion mutants generated in vitro.Gene. 1978 Apr;3(2):87-95 PMID: 350713
-
Active efflux of tetracycline encoded by four genetically different tetracycline resistance determinants in Escherichia coli.Proc Natl Acad Sci U S A. 1980 Jul;77(7):3974-7 PMID: 7001450
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