Rapid qualitative method for detecting staphylococcal nuclease in foods.

Applied and environmental microbiology ·Vol. 35 ·No. 6 ·1978-06-00 ·Pages 1193-7

Koupal A, Deibel RH

Abstract

A rapid method for the detection of heat-stable staphylococcal nuclease in foods is described. The procedure consists of an acid precipitation, boiling, and centrifugation followed by enzyme detection in an agar plate containing deoxyribonucleic acid. To test the efficacy of the procedure, purified Staphylococcus aureus nuclease was added to various foods and recovery experiments were performed. Additionally, foods were inoculated and incubated with S. aureus, and the staphylococcal counts were compared with nuclease activity. The results indicate that the procedure possesses merit for a rapid method that can be incorporated into quality control programs. The procedure requires approximately 2.5 h, and it will detect nuclease levels as low as 10 ng/g of food.

MeSH Terms

Dairy Products/analysis Food Analysis/methods Food Contamination/analysis Food Microbiology Hot Temperature Meat Products/analysis Micrococcal Nuclease/analysis,metabolism Staphylococcus aureus/isolation & purification

Chemicals

Micrococcal Nuclease

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Koupal A

Deibel R H

References (13)

13 references, click to expand

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Synthesis of staphylococcal enterotoxin A and nuclease under controlled fermentor conditions.
Appl Environ Microbiol. 1976 Feb;31(2):243-8 PMID: 11744
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Appl Microbiol. 1974 Oct;28(4):628-37 PMID: 4213939
Applicability of heat-stable deoxyribonuclease assay for assessment of staphylococcal growth and the likely presence of enterotoxin in cheese.
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Metachromatic agar-diffusion microslide technique for detecting staphylococcal nuclease in foods.
Appl Microbiol. 1972 Jan;23(1):168-9 PMID: 4621793
Effect of pH and oxygen tension on staphylococcal growth and enterotoxin formation in fermented sausage.
Appl Microbiol. 1972 Dec;24(6):891-8 PMID: 4631103
Identification of Staphylococcus aureus by simultaneous use of tube coagulase and thermonuclease tests.
Appl Microbiol. 1973 Mar;25(3):496-7 PMID: 4572898
Production and heat stability of staphylococcal nuclease.
Appl Microbiol. 1973 Mar;25(3):332-6 PMID: 4698860
Relationships among coagulase, enterotoxin, and heat-stable deoxyribonuclease production by Staphylococcus aureus.
Appl Microbiol. 1969 Jul;18(1):126-7 PMID: 4896099
Effect of starter culture on staphylococcal enterotoxin and thermonuclease production in dry sausage.
Appl Environ Microbiol. 1976 Jan;31(1):11-20 PMID: 942200
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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.35.6.1193-1197.1978

Published

1978-06-00

Pages

1193-7

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC243004

Subset

External Links

PubMed source DOI: 10.1128/aem.35.6.1193-1197.1978 PMC Full Text View journal details

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Rapid qualitative method for detecting staphylococcal nuclease in foods.

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