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PMID: 6777754 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Effect of DNA length on the nucleosome low salt transition.

Nucleic acids research ·Vol. 8 ·No. 11 ·1980-06-11 ·Pages 2475-87

Dieterich AE, Eshaghpour H, Crothers DM, Cantor CR

Abstract

The effect of DNA length on the low salt unfolding transition of nucleosomes has been studied by the use of fluorescently labeled histones. Nucleosomes were formed by the reconstitution of bulk DNA fragments averaging 173 and 250 base pairs in length. These nucleosomes exhibited a conformational change in a transition centered at about 7 mM ionic strength, very different from that observed for the standard 145 bp nucleosomes (1-3mM). In addition, the conformational change of the 173 and 250 bp nucleosomes involves twice as many ions as that of the 145 bp nucleosomes.

MeSH Terms
Animals Chickens DNA/metabolism Edetic Acid Fluorescent Dyes Histones/metabolism Iodoacetamide/analogs & derivatives Molecular Conformation Naphthalenesulfonates Nucleosomes/metabolism Osmolar Concentration Tromethamine
Chemicals
Fluorescent Dyes Histones Naphthalenesulfonates Nucleosomes Tromethamine 1,5-I-AEDANS DNA Edetic Acid Iodoacetamide
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Dieterich A E
Eshaghpour H
Crothers D M
Cantor C R
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.11.2475
Published
1980-06-11
Pages
2475-87
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324095
Subset
IM
Grants
NIGMS NIH HHS · GM 14825 · United States
NIGMS NIH HHS · GM 21966 · United States
External Links
PubMed source DOI: 10.1093/nar/8.11.2475 PMC Full Text View journal details
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