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PMID: 6772575 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Immunobead enzyme-linked immunosorbent assay for quantitating immunoglobulin A in human secretions and serum.

Infection and immunity ·Vol. 29 ·No. 1 ·1980-07-00 ·Pages 281-3

Sack DA, Neogi PK, Alam MK

Abstract

We have developed an enzyme-linked immunosorbent assay for quantitating human immunoglobulin A in human secretions and serum. The procedure uses Immunobeads as the solid phase, has a sensitivity of 10 ng/ml, and can be completed in 1 day. Since there is no diffusion through agar, the assay does not require a correction factor when measuring secretory immunoglobulin A. This method should be useful in studies of local mucosal immunity in humans, especially when a very sensitive assay is needed.

MeSH Terms

Cholera/immunology Enzyme-Linked Immunosorbent Assay Feces/immunology Female Humans Immunodiffusion Immunoglobulin A/analysis Immunoglobulin A, Secretory/analysis Intestinal Mucosa/immunology Milk, Human/immunology Saliva/immunology Therapeutic Irrigation

Chemicals

Immunoglobulin A Immunoglobulin A, Secretory

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Sack D A

Neogi P K

Alam M K

References (6)

6 references, click to expand

Immunochemical quantitation of antigens by single radial immunodiffusion.
Immunochemistry. 1965 Sep;2(3):235-54 PMID: 4956917
Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies.
Anal Biochem. 1966 Apr;15(1):45-52 PMID: 5959431
Automated immunochemical procedures for measurement of immunoglobulins IgG, IgA, and IgM in human serum.
Clin Chem. 1971 Sep;17(9):936-40 PMID: 4999030
Studies on the quantitation of immunoglobulin in human intestinal secretions.
Gut. 1973 Aug;14(8):616-26 PMID: 4582728
Enzyme-linked immunosorbent assays for cholera serology.
Infect Immun. 1973 May;7(5):759-63 PMID: 4202959
A sensitive method for specific quantitation of secretory IgA.
Scand J Immunol. 1977;6(12):1275-82 PMID: 24264

Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.29.1.281-283.1980

Published

1980-07-00

Pages

281-3

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC551109

Subset

External Links

PubMed source DOI: 10.1128/iai.29.1.281-283.1980 PMC Full Text View journal details

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