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PMID: 6769813 Published · ppublish English Journal Article

Role of antibody and complement in the control of Encephalitozoon cuniculi infections by rabbit macrophages.

Infection and immunity ·Vol. 27 ·No. 3 ·1980-03-00 ·Pages 995-1002

Niederkorn JY, Shadduck JA

Abstract

The capacity of mononuclear peritoneal macrophages to phagocytose Encephalitozoon cuniculi was tested in vitro. Normal rabbit serum or cell culture medium had little effect on the rate of removal of organisms by rabbit peritoneal macrophages. Treatment with immune rabbit serum or immune rabbit immunoglobulin G significantly (P less than 0.001) increased phagocytosis of E. cuniculi. Guinea pig complement was found to significantly (P less than 0.001) enhance the phagocytosis of antibody-treated E. cuniculi. With few exceptions, induced (peritoneal exudate) macrophages were no more effective than unstimulated (resident) macrophages in the phagocytosis of E. cuniculi. Secondary lysosomes labeled with ferritin were seen fusing with phagosomes containing immune rabbit serum-treated parasites. Phagosome-lysosome fusion was not observed when parasites were treated with either normal rabbit serum or culture medium. The results of the present study suggest a role for antibody enhancement of phagocytosis and intracellular killing as a mechanism of resistance to encephalitozoonosis in rabbits.

MeSH Terms
Animals Antibodies/immunology Apicomplexa/immunology,metabolism Complement System Proteins/immunology Lysosomes/ultrastructure Macrophages/immunology,parasitology,ultrastructure Phagocytosis Rabbits Vacuoles/ultrastructure
Chemicals
Antibodies Complement System Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Niederkorn J Y
Shadduck J A
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31 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.27.3.995-1002.1980
Published
1980-03-00
Pages
995-1002
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC550872
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.27.3.995-1002.1980 PMC Full Text View journal details
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