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PMID: 6755395 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

cis 2-Methylthio-ribosylzeatin (ms2io6A) is present in the transfer RNA of Salmonella typhimurium, but not Escherichia coli.

Nucleic acids research ·Vol. 10 ·No. 18 ·1982-09-25 ·Pages 5649-62

Buck M, McCloskey JA, Basile B, Ames BN

Abstract

We have identified the cis isomer of N6-(4-hydroxy-isopentenyl)-2-methylthioadenosine (ms2io6A) as a component of the tRNA of Salmonella typhimurium. This is the first report of this compound in the tRNA of any member of the enterobacteriaceae: the nucleoside was previously thought to be found exclusively in plants or plant associated bacteria. Interestingly, all E. coli strains examined were found to lack ms2io6A. Evidence is presented which suggests S. typhimurium tRNA also contains low levels of 5-carboxymethylaminomethyl-2-thiouridine (cmnm5s2U) in addition to 5-methylaminomethyl-2-thiouridine (mnm5s2U).

MeSH Terms
Adenosine/analogs & derivatives Chromatography, High Pressure Liquid Chromatography, Thin Layer Escherichia coli/analysis Isopentenyladenosine/analogs & derivatives,analysis RNA, Transfer/isolation & purification Salmonella typhimurium/analysis Species Specificity
Chemicals
N(6)-(4-hydroxyisopentenyl)-2-methylthioadenosine Isopentenyladenosine RNA, Transfer Adenosine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Buck M
McCloskey J A
Basile B
Ames B N
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22 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.18.5649
Published
1982-09-25
Pages
5649-62
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320913
Subset
IM
Grants
CGH CDC HHS · GH 21548 · United States
NIGMS NIH HHS · GM-19993 · United States
External Links
PubMed source DOI: 10.1093/nar/10.18.5649 PMC Full Text View journal details
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