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PMID: 6754621 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Sensitization or tolerance to Mycobacterium leprae antigen by route of injection.

Infection and immunity ·Vol. 38 ·No. 2 ·1982-11-00 ·Pages 673-80

Shepard CC, Walker LL, Van Landingham RM, Ye SZ

Abstract

Aqueous suspensions of heat-killed Mycobacterium leprae in a dose of 10(7) organisms were highly immunogenic when injected intradermally (i.d.). The same dose of bacteria did not sensitize when given intraperitoneally (i.p.) or intravenously (i.v.), and did so only minimally at best when given subcutaneously. The i.d. route was the most immunogenic for sheep erythrocytes also. M. leprae injected i.p. or i.v. stimulated immune tolerance to M. leprae challenge i.d. In older mice (greater than or equal to 8 weeks), the i.v. injections gave more complete tolerance. Mice that had been rendered tolerant by i.v. injections maintained their tolerance for at least 168 days. Prior UV irradiation of intact mice prevented sensitization by the i.d. route. In normal mice, living M. bovis BCG given i.d. produced good sensitization to M. leprae. Mice that had been made tolerant by i.v. injection of M. leprae could be partially sensitized to M. leprae by i.d. immunization with BCG; mixtures of living BCG and heat-killed M. leprae were no more effective than BCG alone. These findings appear to have relevance to the pathogenesis of lepromatous leprosy and its immunoprophylaxis.

MeSH Terms
Animals BCG Vaccine/immunology Female Hypersensitivity, Delayed Immune Tolerance Immunization Injections, Intradermal Injections, Intraperitoneal Injections, Intravenous Injections, Subcutaneous Mice Mice, Inbred C57BL Mice, Inbred CBA Mycobacterium leprae/immunology Ultraviolet Rays
Chemicals
BCG Vaccine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Shepard C C
Walker L L
Van Landingham R M
Ye S Z
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31 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.38.2.673-680.1982
Published
1982-11-00
Pages
673-80
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC347791
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.38.2.673-680.1982 PMC Full Text View journal details
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