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PMID: 6751327 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Differential effects of Ca2+-calmodulin on adenylate cyclase activity cyclase activity in mouse and rat pancreatic islets.

The Biochemical journal ·Vol. 206 ·No. 1 ·1982-07-15 ·Pages 97-102

Thams P, Capito K, Hedeskov CJ

Abstract

The effects of Ca2+-calmodulin on adenylate cyclase activity in EGTA-washed, 27000 g particulate fractions of mouse and rat pancreatic islets were studied. Ca2+ (10 microM)-calmodulin (1 microM) stimulated adenylate cyclase activity 53.1 +/- 5.2 (N = 6)% in the particulate fraction of rat islets. Trifluoperazine (50 microM), a specific inhibitor of calmodulin, inhibited the Ca2+-calmodulin activation of the adenylate cyclase activity of this fraction of rat islets. These results confirm previous reports dealing with Ca2+-Calmodulin and rat islet adenylate cyclase [Valverde, Vandermeers. Anjaneyulu & Malaisse (1979) Science 206, 225-227; Sharp, Wiedenkeller, Kaelin, Siegel & Wollheim (1980) Diabetes 29, 74-77]. In contrast, however, Ca2+ (1-100 microM)-calmodulin (1-10 microM) did not stimulate the adenylate cyclase activity in the EGTA-washed particulate fraction of mouse islets, and trifluoperazine (50 microM) did not inhibit the adenylate cyclase activity of this fraction of mouse islets, although some remaining calmodulin [0.18 +/- 0.05 (n = 3) microgram/mg of protein] could be demonstrated. GTP (10 microM) enhanced islet adenylate cyclase activity considerably, but did not confer any sensitivity towards Ca2+-calmodulin on mouse islet adenylate cyclase. The results question the role of calmodulin in the Ca2+-dependent rise in cyclic AMP evoked by glucose in pancreatic islets.

MeSH Terms
Adenylyl Cyclases/metabolism Animals Calcium/pharmacology Calcium-Binding Proteins/pharmacology Calmodulin/pharmacology Dose-Response Relationship, Drug Enzyme Activation/drug effects Guanosine Triphosphate/pharmacology In Vitro Techniques Islets of Langerhans/drug effects,enzymology Male Mice Rats Species Specificity Trifluoperazine/pharmacology
Chemicals
Calcium-Binding Proteins Calmodulin Trifluoperazine Guanosine Triphosphate Adenylyl Cyclases Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Thams P
Capito K
Hedeskov C J
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25 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2060097
Published
1982-07-15
Pages
97-102
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1158554
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2060097 PMC Full Text View journal details
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