Reformation of organized epidermal structure by transplantation of suspensions and cultures of epidermal and dermal cells.

Cell and tissue research ·Vol. 225 ·No. 1 ·1982-00-00 ·Pages 65-77

Worst PK, Mackenzie IC, Fusenig NE

Abstract

The development of epidermis and epidermal appendages from dissociated cells of neonate mouse skin was examined by transplantation of cell suspensions to subdermally prepared, protected graft beds. Using Ficol gradients and culture procedures, we prepared subfractions of primary cell suspensions consisting of essentially pure epidermal cells or fibroblasts. Reformation of an epithelium structurally similar to the epidermis was observed from transplanted epidermal-cell suspensions, but formation of hair follicles and development of normal epidermal microarchitecture was observed only when epidermal cells were transplanted together with cells of dermal origin. This pattern was observed following transplantation of either fresh-cell isolates or cells cultured up to 7 days prior to transplantation.

MeSH Terms

Animals Cell Differentiation Cell Separation Cells, Cultured Collagen Connective Tissue Cells Epidermal Cells Epidermis/transplantation Epithelial Cells Female Male Mice Mice, Inbred C3H Skin/cytology Skin Transplantation

Chemicals

Collagen

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Worst P K

Mackenzie I C

Fusenig N E

References (27)

27 references, click to expand

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Article Info

Journal

Cell and tissue research

Abbr.

Cell Tissue Res

ISSN

0302-766X

DOI

10.1007/BF00216219

Published

1982-00-00

Pages

65-77

Language

English

Region

Germany

NLM ID

0417625

Subset

External Links

PubMed source DOI: 10.1007/BF00216219 View journal details

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Reformation of organized epidermal structure by transplantation of suspensions and cultures of epidermal and dermal cells.

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