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PMID: 6728676 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

In situ activation of bis-dialkylaminophosphines--a new method for synthesizing deoxyoligonucleotides on polymer supports.

Nucleic acids research ·Vol. 12 ·No. 10 ·1984-05-25 ·Pages 4051-61

Barone AD, Tang JY, Caruthers MH

Abstract

Deoxynucleoside phosphoramidites can be prepared in good yield from deoxynucleosides, bis- dialkylaminophosphines , and the corresponding dialkylamine hydrotetrazolide or tetrazole as catalysts. These phosphoramidites generated in situ lead to the direct synthesis of deoxyoligonucleotides on polymer supports.

MeSH Terms

Indicators and Reagents Magnetic Resonance Spectroscopy/methods Mass Spectrometry/methods Oligodeoxyribonucleotides/chemical synthesis Oligonucleotides/chemical synthesis Organophosphorus Compounds Solvents

Chemicals

Indicators and Reagents Oligodeoxyribonucleotides Oligonucleotides Organophosphorus Compounds Solvents

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Barone A D

Tang J Y

Caruthers M H

References (2)

2 references, click to expand

Synthesis of oligodeoxyribonucleotides on silica gel support.
Nucleic Acids Res. 1981 Jun 25;9(12):2807-17 PMID: 6269061
Restriction and modification of a self-complementary octanucleotide containing the EcoRI substrate.
J Mol Biol. 1975 Dec 5;99(2):237-61 PMID: 173854

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/12.10.4051

Published

1984-05-25

Pages

4051-61

Language

English

Region

England

NLM ID

0411011

PMCID

PMC318815

Subset

Grants

NIGMS NIH HHS · GM25680 · United States

External Links

PubMed source DOI: 10.1093/nar/12.10.4051 PMC Full Text View journal details

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In situ activation of bis-dialkylaminophosphines--a new method for synthesizing deoxyoligonucleotides on polymer supports.

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