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PMID: 6728453 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The tubulins of animals, plants, fungi and protists implications for metazoan evolution.

Origins of life ·Vol. 13 ·No. 3-4 ·1984-03-00 ·Pages 169-76

Little M, Ludueña RF, Morejohn LC, Asnes C, Hoffman E

Abstract

alpha-Tubulin subunits from trout (S. gairdneri) sperm tails, sea urchin (S. purpuratus) cilia, protistan alga (C. elongatum ) flagella and rose (Paul's Scarlet) cytoplasm have been characterized by limited proteolytic cleavage with the enzyme Staphylococcus aureus protease and electrophoresis of the digestion products on SDS-PAGE. The resulting patterns corresponded to either of two major types representative of animal and non-animal alpha-tubulins, respectively. A total of 28 alpha-tubulins have now been characterized by this method. They are classified in this paper according to the type of cleavage pattern generated by the enzyme S. aureus protease. The implications of these results for metazoan evolution are discussed.

MeSH Terms
Animals Biological Evolution Cilia/analysis Eukaryota/analysis Flagella/analysis Macromolecular Substances Male Peptide Fragments/analysis Sea Urchins/analysis Species Specificity Spermatozoa/analysis Trout Tubulin/analysis
Chemicals
Macromolecular Substances Peptide Fragments Tubulin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Little M
Ludueña R F
Morejohn L C
Asnes C
Hoffman E
References (18)
18 references, click to expand
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Article Info
Journal
Origins of life
Abbr.
Orig Life
ISSN
0302-1688
Published
1984-03-00
Pages
169-76
Language
English
Region
Netherlands
NLM ID
0420542
Subset
IM
External Links
PubMed source
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