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PMID: 6725393 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and characterization of a high molecular weight actin-binding protein from Physarum polycephalum plasmodia.

The Journal of cell biology ·Vol. 98 ·No. 5 ·1984-05-00 ·Pages 1611-8

Sutoh K, Iwane M, Matsuzaki F, Kikuchi M, Ikai A

Abstract

A high molecular weight actin-binding protein was isolated from the Physarum polycephalum plasmodia. The protein ( HMWP ) shares many properties with other high molecular weight actin-binding proteins such as spectrin, actin-binding protein from macrophages, and filamin. It has a potent activity to cross-link F-actin into a gel-like structure. Its cross-linking activity does not depend on calcium concentrations. Hydrodynamic studies have revealed that the protein is in the monomeric state of a polypeptide chain with molecular weight of approximately 230,000 in a high ionic strength solvent, while it self-associates into a dimer under physiological ionic conditions. Electron microscopic examinations of HMWP have shown that the monomer particle observed in a high ionic strength solvent is rod shaped with the two-stranded morphology very similar to that of spectrin. On the other hand, under physiological ionic conditions, the HMWP dimer shows the dumb-bell shape with two globular domains connected with a thin flexible strand.

MeSH Terms
Actins/metabolism Amino Acids/analysis Carrier Proteins/isolation & purification Gelsolin Macromolecular Substances Microfilament Proteins Microscopy, Electron Molecular Weight Physarum/analysis Protein Binding Protein Conformation
Chemicals
Actins Amino Acids Carrier Proteins Gelsolin Macromolecular Substances Microfilament Proteins brevin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sutoh K
Iwane M
Matsuzaki F
Kikuchi M
Ikai A
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27 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.98.5.1611
Published
1984-05-00
Pages
1611-8
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2113185
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.98.5.1611 PMC Full Text View journal details
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