Ubiquinone-mediated coupling of NADH dehydrogenase to active transport in membrane vesicles from Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 10 ·1975-10-00 ·Pages 3970-4

Stroobant P, Kaback HR

Abstract

Addition of ubiquinone-1 to E. coli ML 308-225 membrane vesicles dramatically increases coupling between NADH oxidation and active transport such that initial rates and steady-state levels of lactose and amino-acid accumulation are comparable to those observed during D-lactate oxidation. Similar but less dramatic effects are observed with the quinone and succinate or L-lactate. In the presence of NADH and ubiquinone-1, the vesicles also generate a membrane potential (interior negative) that is similar in magnitude to that observed in the presence of D-lactate. Stimulation of NADH-dependent transport by ubiquinone-1 cannot be accounted for by increased rates of oxidation of NADH, and the effect of the quinone on NADH-dependent lactose transport is not observed in vesicles depleted of NADH dehydrogenase activity. Thus, it is apparent that ubiquinone-1 shunts electrons from NADH dehydrogenase [NADH:(acceptor)oxidoreductase; EC 1.6.99.3] to the portion of the respiratory chain containing the energy-coupling site. The findings demonstrate unequivocally that inefficient coupling of NADH oxidation to active transport cannot be due to the presence of inverted vesicles. In addition, they provide further support for specific localization of the energy-coupling site.

MeSH Terms

Amino Acids/metabolism Biological Transport, Active Cell Membrane/drug effects,enzymology Escherichia coli/drug effects,enzymology,metabolism Kinetics Lactates/metabolism Lactose/metabolism NAD/metabolism NADH, NADPH Oxidoreductases/metabolism NADP/metabolism Oxygen Consumption/drug effects Succinates/metabolism Ubiquinone/pharmacology

Chemicals

Amino Acids Lactates Succinates NAD Ubiquinone NADP NADH, NADPH Oxidoreductases Lactose

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Stroobant P

Kaback H R

References (30)

30 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.72.10.3970

Published

1975-10-00

Pages

3970-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433119

Subset

External Links

PubMed source DOI: 10.1073/pnas.72.10.3970 PMC Full Text View journal details

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Ubiquinone-mediated coupling of NADH dehydrogenase to active transport in membrane vesicles from Escherichia coli.

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