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PMID: 6717433 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Accelerated onset of viral transcription in adenovirus-infected HeLa cells treated with the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate.

Molecular and cellular biology ·Vol. 4 ·No. 3 ·1984-03-00 ·Pages 563-6

Carter TH, Milovanovic ZZ, Babiss LE, Fisher PB

Abstract

When adenovirus type 5-infected HeLa cells were exposed to the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate, short pulse-labeling with [3H]uridine in vivo and [3H]UTP incorporation by isolated nuclei in vitro were both consistent with a decreased latent period before initiation by RNA polymerase at early viral promoters. Acceleration was not dependent upon concurrent protein synthesis and could not be attributed to rapid entry of virus into the cell nucleus. 12-O-tetradecanoyl-phorbol-13-acetate suppressed the transcription-delay phenotype of the E1a mutant, hr1, without restoring its ability to replicate.

MeSH Terms
Adenoviruses, Human/drug effects,genetics Cell Nucleus/drug effects,metabolism Cell Transformation, Viral Cycloheximide/pharmacology DNA Replication/drug effects DNA, Viral/genetics HeLa Cells/drug effects,metabolism Humans Kinetics Phorbols/toxicity Tetradecanoylphorbol Acetate/toxicity Transcription, Genetic/drug effects
Chemicals
DNA, Viral Phorbols Cycloheximide Tetradecanoylphorbol Acetate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Carter T H
Milovanovic Z Z
Babiss L E
Fisher P B
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33 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.3.563-566.1984
Published
1984-03-00
Pages
563-6
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368737
Subset
IM
Grants
NIAID NIH HHS · AI-12052 · United States
NCI NIH HHS · CA-25757 · United States
External Links
PubMed source DOI: 10.1128/mcb.4.3.563-566.1984 PMC Full Text View journal details
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