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PMID: 671585 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Effect of UV irradiation on the expression of vaccinia virus gene products synthesized in a cell-free system coupling transcription and translation.

Journal of virology ·Vol. 26 ·No. 3 ·1978-06-00 ·Pages 673-80

Bossart W, Nuss DL, Paoletti E

Abstract

The effect of UV irradiation on the expression of the vaccinia virus genome was investigated in a cell-free system coupling transcription with translation. Exposure of vaccinia virus to an increasing dose of irradiation resulted in differential reduction in the syntheses of virus-specified polypeptides in the coupled system, with sensitivity being proportional to the size of the gene pro duct. This suggests that each translationally functional mRNA species produced in vitro by vaccinia virus cores is synthesized from an individual promoter site.

MeSH Terms

Cell-Free System Peptide Biosynthesis Protein Biosynthesis/radiation effects RNA, Messenger/biosynthesis RNA, Viral/biosynthesis Transcription, Genetic/radiation effects Ultraviolet Rays Vaccinia virus/metabolism,radiation effects Viral Proteins/biosynthesis

Chemicals

RNA, Messenger RNA, Viral Viral Proteins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Bossart W

Nuss D L

Paoletti E

References (15)

15 references, click to expand

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The definition of a large viral transcription unit late in Ad2 infection of HeLa cells: mapping by effects of ultraviolet irradiation.
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In vitro synthesis of a high molecular weight virion-associated RNA by vaccinia.
J Biol Chem. 1977 Feb 10;252(3):866-71 PMID: 838701
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Proc Natl Acad Sci U S A. 1974 Aug;71(8):3014-8 PMID: 4606808
Sequential transcription of the genes of vesicular stomatitis virus.
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Characterization and localization of the naturally occurring cross-links in vaccinia virus DNA.
J Mol Biol. 1974 Oct 5;88(4):785-96 PMID: 4427382
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J Virol. 1973 Aug;12(2):241-52 PMID: 4747985
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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.26.3.673-680.1978

Published

1978-06-00

Pages

673-80

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC525891

Subset

External Links

PubMed source DOI: 10.1128/JVI.26.3.673-680.1978 PMC Full Text View journal details

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Effect of UV irradiation on the expression of vaccinia virus gene products synthesized in a cell-free system coupling transcription and translation.

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