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PMID: 6713072 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Stiffness of glycerinated rabbit psoas fibers in the rigor state. Filament-overlap relation.

Biophysical journal ·Vol. 45 ·No. 3 ·1984-03-00 ·Pages 593-602

Tawada K, Kimura M

Abstract

The stiffness of glycerinated rabbit psoas fibers in the rigor state was measured at various sarcomere lengths in order to determine the distribution of the sarcomere compliance between the cross-bridge and other structures. The stiffness was determined by measuring the tension increment at one end of a fiber segment while stretching the other end of the fiber. The contribution of the end compliance to the rigor segments was checked both by laser diffractometry of the sarcomere length change and by measuring the length dependence of the Young's modulus; the contribution was found to be small. The stiffness in the rigor state was constant at sarcomere lengths of 2.4 microns or less; at greater sarcomere lengths the stiffness, when corrected for the contribution of resting stiffness, scaled with the amount of overlap between the thick and thin filaments. These results suggest that the source of the sarcomere compliance of the rigor fiber at the full overlapping of filaments is mostly the cross-bridge compliance.

MeSH Terms
Animals Elasticity Glycerol In Vitro Techniques Lasers Muscle Contraction Muscle Relaxation Muscle Rigidity/physiopathology Muscles/physiopathology Rabbits Sarcomeres/physiology Transducers
Chemicals
Glycerol
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tawada K
Kimura M
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28 references, click to expand
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(84)84197-1
Published
1984-03-00
Pages
593-602
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1434884
Subset
IM
External Links
PubMed source DOI: 10.1016/S0006-3495(84)84197-1 PMC Full Text View journal details
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