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PMID: 6712612 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cleavage of formate from omega,4-dihydroxyacetophenone. An unusual oxygen-requiring reaction in the bacterial catabolism of 4-hydroxyacetophenone.

The Biochemical journal ·Vol. 218 ·No. 1 ·1984-02-15 ·Pages 269-72

Hopper DJ, Elmorsi EA

Abstract

An enzyme, from a soil bacterium grown on 4-hydroxyacetophenone, cleaved the side chain of omega,4-dihydroxyacetophenone between the keto group and the carbon atom bearing a hydroxy group to give 4-hydroxybenzoate and formate. The reaction was O2-dependent. Partially purified enzyme required no added cofactors for activity.

MeSH Terms
Acetophenones/metabolism Alcaligenes/metabolism Chromatography, DEAE-Cellulose Formates/metabolism Oxygen/metabolism Spectrophotometry
Chemicals
Acetophenones Formates formic acid 4-hydroxyacetophenone Oxygen
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hopper D J
Elmorsi E A
References (10)
10 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2180269
Published
1984-02-15
Pages
269-72
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1153332
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2180269 PMC Full Text View journal details
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