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PMID: 6699079 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

To grow mouse mammary epithelial cells in culture.

The Journal of cell biology ·Vol. 98 ·No. 3 ·1984-03-00 ·Pages 1026-32

Ehmann UK, Peterson WD, Misfeldt DS

Abstract

Normal mouse mammary epithelial cells from Balb/c mice were successfully cultivated on tissue culture plastic with lethally irradiated LA7 feeder cells. The feeder cells also promoted colony formation from single mouse mammary cells, and the fraction of cells that formed colonies was proportional to the density of feeder cells. The mouse mammary cells could be passaged at least 8-12 times as long as new feeder cells were added at each passage. The cells now in culture have doubled in number at least 30 times, but the in vitro lifespan is not yet known. The cultures of mouse cells maintained by this technique never became overgrown with fibroblasts and numerous domes formed in the cultures.

MeSH Terms
Animals Cell Division Cells, Cultured Epithelial Cells Female Mammary Glands, Animal/cytology,ultrastructure Mice Microscopy, Electron
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ehmann U K
Peterson W D
Misfeldt D S
References (33)
33 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.98.3.1026
Published
1984-03-00
Pages
1026-32
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2113157
Subset
IM
Grants
NCI NIH HHS · N01-CB-74094 · United States
NCI NIH HHS · N01-CP-21017 · United States
External Links
PubMed source DOI: 10.1083/jcb.98.3.1026 PMC Full Text View journal details
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