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PMID: 6696736 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

16S acetylcholinesterase of the extracellular matrix is assembled within mouse muscle cells in culture.

The Biochemical journal ·Vol. 217 ·No. 2 ·1984-01-15 ·Pages 377-81

Inestrosa NC

Abstract

The present paper examines where the extracellular-matrix (ECM) 16S acetylcholinesterase (AChE, EC 3.1.1.7) is assembled in muscle cells in culture. The existence of an internal pool of 16S AChE was detected by using AChE inhibitors of differing membrane permeability. After irreversible inhibition of all cellular esterase, the newly synthesized 16S form appears in an intracellular compartment and is only later detected on the cell surface. Results show that the ECM 16S AChE is assembled within muscle cells.

MeSH Terms

Acetylcholinesterase/biosynthesis Animals Cell Compartmentation Cell Line Cells, Cultured Cholinesterase Inhibitors/pharmacology Extracellular Matrix/enzymology Isoflurophate/pharmacology Mice Muscles/cytology,drug effects,enzymology

Chemicals

Cholinesterase Inhibitors Isoflurophate Acetylcholinesterase

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Inestrosa N C

References (14)

14 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2170377

Published

1984-01-15

Pages

377-81

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1153227

Subset

External Links

PubMed source DOI: 10.1042/bj2170377 PMC Full Text View journal details

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16S acetylcholinesterase of the extracellular matrix is assembled within mouse muscle cells in culture.

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