An essential role for glucocorticoid in casein gene expression in rat mammary explants.

Biochemical and biophysical research communications ·Vol. 114 ·No. 1 ·1983-07-18 ·Pages 380-7

Kulski JK, Topper YJ, Chomczynski P, Qasba P

Abstract

It is demonstrated that the accumulation of 42 K casein mRNA in mammary tissue from adrenalectomized, virgin rats is almost 20x higher in the presence of exogenous hydrocortisone than in its absence. Accumulation of 25 K casein mRNA in this tissue is totally dependent on the steroid. The results indicate a much greater dependency on hydrocortisone than was appreciated previously, and also show that this dependency does not reflect a loss of cell viability in the absence of the steroid.

MeSH Terms

Adrenalectomy Animals Caseins/genetics DNA/metabolism Female Genes/drug effects Hydrocortisone/pharmacology Kinetics Mammary Glands, Animal/metabolism Nucleic Acid Hybridization Organ Culture Techniques Plasmids Protein Biosynthesis RNA, Messenger/genetics Rats Rats, Inbred Strains Transcription, Genetic/drug effects

Chemicals

Caseins RNA, Messenger DNA Hydrocortisone

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Kulski J K

Topper Y J

Chomczynski P

Qasba P

Article Info

Journal

Biochemical and biophysical research communications

Abbr.

Biochem Biophys Res Commun

ISSN

0006-291X

DOI

10.1016/0006-291x(83)91638-8

Published

1983-07-18

Pages

380-7

Language

English

Region

United States

NLM ID

0372516

Subset

External Links

PubMed source DOI: 10.1016/0006-291x(83)91638-8 View journal details

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An essential role for glucocorticoid in casein gene expression in rat mammary explants.

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