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PMID: 6687939 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Chromosomal mapping of Xenopus 5S genes: somatic-type versus oocyte-type.

Nucleic acids research ·Vol. 11 ·No. 8 ·1983-04-25 ·Pages 2313-23

Harper ME, Price J, Korn LJ

Abstract

Xenopus 5S RNA genes exhibit a pattern of differential expression during development in which some members (oocyte-type) are transcribed only in oocytes, while others (somatic-type) are expressed in both oocytes and somatic cells. Using cloned DNA probes specific for each gene type, we determined the positions of these genes on Xenopus metaphase chromosomes by in situ hybridization. Somatic-type 5S genes in both X. laevis and X. borealis are located at the distal end of the long arm of only one chromosome (number 9). The oocyte-type 5S RNA genes are found at the distal ends of the long arms of most Xenopus chromosomes, including chromosome 9. Thus, large scale differences in chromosomal location cannot explain the selective expression of these genes, as suggested previously.

MeSH Terms
Animals Chromosome Mapping DNA/analysis,genetics DNA, Ribosomal Female Genes Karyotyping Metaphase Nucleic Acid Hybridization Oocytes/physiology Ovum/physiology Species Specificity Transcription, Genetic Xenopus
Chemicals
DNA, Ribosomal DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Harper M E
Price J
Korn L J
References (25)
25 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.8.2313
Published
1983-04-25
Pages
2313-23
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325886
Subset
IM
Grants
NIGMS NIH HHS · GM 07992 · United States
NIGMS NIH HHS · GM 29797 · United States
External Links
PubMed source DOI: 10.1093/nar/11.8.2313 PMC Full Text View journal details
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