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PMID: 6687410 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Protein sequence analysis: automated microsequencing.

Science (New York, N.Y.) ·Vol. 219 ·No. 4585 ·1983-02-11 ·Pages 650-9

Hunkapiller MW, Hood LE

Abstract

The automated microsequencing of proteins can now be carried out at the 5- to 10-picomoles (submicrogram) level on polypeptides obtained directly from one- and two-dimensional gel electrophoresis. The techniques are applicable to polypeptides ranging in size from small peptides (less than 10 residues) to large proteins (more than 1000 residues).

MeSH Terms
Amino Acid Sequence Autoanalysis/instrumentation DNA/genetics Peptides/analysis Proteins/analysis
Chemicals
Peptides Proteins DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hunkapiller M W
Hood L E
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
0036-8075
DOI
10.1126/science.6687410
Published
1983-02-11
Pages
650-9
Language
English
Region
United States
NLM ID
0404511
Subset
IM
Grants
NIGMS NIH HHS · GM 06965 · United States
External Links
PubMed source DOI: 10.1126/science.6687410 View journal details
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