A steady efflux of ionic current predicts hind limb development in the axolotl.

For more than a week prior to the emergence of a hind limb, a steady electric current is driven out of the ventrolateral flank in the immature axolotl, returning through the integument in adjacent regions of the body. A marked peak in the density of this outcurrent could be observed over the exact area of hind limb formation 4 to 6 days prior to its appearance. After a bud projected from the flank, current densities were observed to decrease in magnitude yet localize about the early limb. In about one-half of the animals observed, current reversed its polarity and entered the apex of large buds, 0.4 to 0.5 mm in length. We discuss the possible role such endogenous currents and their associated fields may play in limb development and compare it to similar current flow associated with the regeneration of amphibian limbs.