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PMID: 6644622 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Evidence for acetylcholine receptor blockade by intracellular hydrogen ions in cultured chick myoballs.

The Journal of physiology ·Vol. 343 ·1983-10-00 ·Pages 429-37

Goldberg G, Lass Y

Abstract

Acetylcholine (ACh)-induced membrane currents were recorded in voltage-clamped myoballs in culture. The ACh sensitivity and the charge transfer per channel (Qc) were doubled in NH4+ at normal extracellular pH (pHo). Immediately after wash-out of NH4+, the ACh sensitivity and Qc undershot below the control. The recovery period of the ACh sensitivity and Qc was about 20 min. The ACh sensitivity and Qc greatly increased at alkaline pHo and decreased at acid pHo. We suggest that the intracellular H+ ions can block the ACh-induced channel. The pHo effects may be mediated by a similar (but smaller) change in the intracellular pH (pHi). Intracellular alkalinization can improve the channel function.

MeSH Terms

Acetylcholine/metabolism Ammonium Chloride/pharmacology Animals Cells, Cultured Chick Embryo Hydrogen-Ion Concentration Intracellular Fluid/metabolism Membrane Potentials Muscles/metabolism Receptors, Cholinergic/drug effects,metabolism,physiology

Chemicals

Receptors, Cholinergic Ammonium Chloride Acetylcholine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Goldberg G

Lass Y

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29 references, click to expand

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Article Info

Journal

The Journal of physiology

Abbr.

J Physiol

ISSN

0022-3751

DOI

10.1113/jphysiol.1983.sp014901

Published

1983-10-00

Pages

429-37

Language

English

Region

England

NLM ID

0266262

PMCID

PMC1193928

Subset

External Links

PubMed source DOI: 10.1113/jphysiol.1983.sp014901 PMC Full Text View journal details

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Evidence for acetylcholine receptor blockade by intracellular hydrogen ions in cultured chick myoballs.

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