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PMID: 6626175 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Neural 16S acetylcholinesterase is solubilized by heparin.

The Biochemical journal ·Vol. 215 ·No. 1 ·1983-10-01 ·Pages 201-4

Torres JC, Behrens MI, Inestrosa NC

Abstract

The effect of heparin, a sulphated glycosaminoglycan, on the solubilization of rat sciatic-nerve acetylcholinesterase (acetylcholine acetylhydrolase; AChE; EC 3.1.1.7) was studied. It was found that heparin solubilized esterase activity from ligated nerves. Sedimentation analysis revealed this activity to be mainly the 16S form. Chondroitin sulphate did not solubilize AChE activity, and protamine eliminated the solubilizing effect. Our results suggest the involvement of sulphated glycosaminoglycans in the intra-axonal localization and transport of 16S AChE.

MeSH Terms
Acetylcholinesterase/metabolism Animals Biological Transport Centrifugation, Density Gradient Heparin/metabolism,pharmacology Male Protamines/pharmacology Rats Rats, Inbred Strains Sciatic Nerve/enzymology Solubility
Chemicals
Protamines Heparin Acetylcholinesterase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Torres J C
Behrens M I
Inestrosa N C
References (22)
22 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2150201
Published
1983-10-01
Pages
201-4
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1152381
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2150201 PMC Full Text View journal details
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