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PMID: 6626169 Published · ppublish English Journal Article

Specific association of iduronic acid-rich dermatan sulphate with the extracellular matrix of human skin fibroblasts cultured on collagen gels.

The Biochemical journal ·Vol. 215 ·No. 1 ·1983-10-01 ·Pages 107-16

Gallagher JT, Gasiunas N, Schor SL

Abstract

Human skin fibroblasts cultured on collagen gels produced two dermatan sulphate species, one, enriched in iduronic acid residues, that bound specifically to the collagenous fibres of the gel, the other, enriched in glucuronic acid, that accumulated in the culture medium. Collagen-binding and collagen-non-binding dermatan sulphates were also produced by cells grown on plastic surfaces, but in these cultures each constituent was released into the growth medium. Net synthesis of dermatan sulphate was 3-fold higher in cells maintained on collagen gels. In contrast, heparan sulphate synthesis was not influenced by the nature of the culture surface. The concentration of heparan sulphate in surface-membrane extracts was similar for cells grown on plastic and on collagen gels, but cells cultured on collagen showed a notable increase in the content of surface-membrane dermatan sulphate. The patterns of synthesis and distribution of sulphated glycosaminoglycans observed in skin fibroblasts maintained on collagen gels may reflect differentiated cellular functions.

MeSH Terms
Biopolymers Cells, Cultured Child Child, Preschool Chondroitin/analogs & derivatives Chromatography, DEAE-Cellulose Collagen Culture Media Dermatan Sulfate/metabolism Extracellular Matrix/metabolism Fibroblasts/metabolism Gels Glucuronates/analysis Glucuronic Acid Glycosaminoglycans/metabolism Humans Iduronic Acid/analysis Male Skin/cytology,metabolism Uronic Acids/analysis
Chemicals
Biopolymers Culture Media Gels Glucuronates Glycosaminoglycans Uronic Acids Dermatan Sulfate Iduronic Acid Glucuronic Acid Chondroitin Collagen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gallagher J T
Gasiunas N
Schor S L
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32 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2150107
Published
1983-10-01
Pages
107-16
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1152369
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2150107 PMC Full Text View journal details
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