Methyltransferases involved in methanol conversion by Methanosarcina barkeri.

Archives of microbiology ·Vol. 134 ·No. 3 ·1983-06-00 ·Pages 238-42

van der Meijden P, Heythuysen HJ, Pouwels A, Houwen F, van der Drift C, Vogels GD

Abstract

2-(Methylthio)ethanesulfonate (CH3S-CoM) is formed as an intermediate in methanogenesis from methanol by cell-free extracts of Methanosarcina barkeri. The enzyme system involved in the methyl transfer from methanol to 2-mercaptoethanesulfonate (HS-CoM) was resolved into two enzyme fractions. One enzyme (methanol:5-hydroxy-benzimidazolylcobamide methyltransferase) appears to be a cobalamin-containing protein, which is oxygen sensitive. The other enzyme (Co-methyl-5-hydroxybenzimidazolylcobamide: HS-CoM methyltransferase) was purified. It is insensitive to oxygen and it transfers also the methylgroup from Co-methyl-5,6-dimethylbenzimidazolylcobamide to HS-CoM.

MeSH Terms

Chromatography, Ion Exchange Euryarchaeota/enzymology Methanol/metabolism Methyltransferases/isolation & purification,metabolism

Chemicals

Methyltransferases Methanol

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

van der Meijden P

Heythuysen H J

Pouwels A

Houwen F

van der Drift C

Vogels G D

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18 references, click to expand

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Article Info

Journal

Archives of microbiology

Abbr.

Arch Microbiol

ISSN

0302-8933

DOI

10.1007/BF00407765

Published

1983-06-00

Pages

238-42

Language

English

Region

Germany

NLM ID

0410427

Subset

External Links

PubMed source DOI: 10.1007/BF00407765 View journal details

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