Stiffness, force, and sarcomere shortening during a twitch in frog semitendinosus muscle bundles.

Biophysical journal ·Vol. 45 ·No. 2 ·1984-02-00 ·Pages 389-97

Schoenberg M, Wells JB

Abstract

The time course of force and stiffness during a twitch was determined at 6 and 26 degrees C in frog semitendinosus muscle bundles using the transmission time technique of Schoenberg, M., J.B. Wells, and R.J. Podolsky, 1974, J. Gen. Physiol. 64:623-642. Sarcomere shortening due to series compliance was also measured using a laser light diffraction technique. Following stimulation, stiffness developed more rapidly than force, but had a slower time course than published Ca2+ transients determined from light signals using Ca2+ sensitive dyes (Baylor, S.M., W.K. Chandler, and M.W. Marshall, 1982, J. Physiol. (Lond.). 331:139-177). Stiffness (S) did not reach its tetanic value during a twitch at 6 or 26 degrees C, although at 6 degrees C, it approached close to this value with S-twitch/S-tetanus = 0.82 +/- 0.07 (+/- SEM). During relaxation, force fell more rapidly than stiffness both for a twitch and also a tetanus. Also in this paper, several of the assumptions inherent in using the transmission time technique for the measurement of stiffness are considered in detail.

MeSH Terms

Animals Biomechanical Phenomena In Vitro Techniques Muscle Contraction Myofibrils/physiology Rana pipiens/physiology Sarcomeres/physiology,ultrastructure Temperature Time Factors

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Schoenberg M

Wells J B

References (28)

28 references, click to expand

Tension responses to sudden length change in stimulated frog muscle fibres near slack length.
J Physiol. 1977 Jul;269(2):441-515 PMID: 302333
The relation between stiffness and filament overlap in stimulated frog muscle fibres.
J Physiol. 1981 Feb;311:219-49 PMID: 6973625
The onset of shortening in striated muscle.
J Physiol. 1951 Apr;113(2-3):336-45 PMID: 14832779
An analysis of the mechanical components in frog's striated muscle.
J Physiol. 1958 Oct 31;143(3):515-40 PMID: 13588571
Two rigor states in skinned crayfish single muscle fibers.
J Gen Physiol. 1976 Sep;68(3):267-80 PMID: 821913
Return of myosin heads to thick filaments after muscle contraction.
Science. 1977 Aug 12;197(4304):685-7 PMID: 301660
Some self-consistent two-state sliding filament models of muscle contraction.
Biophys J. 1975 Apr;15(4):335-72 PMID: 1125390
Inward spread of activation in vertebrate muscle fibres.
J Physiol. 1971 Feb;212(3):777-99 PMID: 5557071
Sarcomere length changes during stimulation of frog semitendinosus muscle.
J Mechanochem Cell Motil. 1976 Mar;3(3):155-61 PMID: 932563
Use of metallochromic dyes to measure changes in myoplasmic calcium during activity in frog skeletal muscle fibres.
J Physiol. 1982 Oct;331:139-77 PMID: 6984070
Tension due to interaction between the sliding filaments in resting striated muscle. The effect of stimulation.
J Physiol. 1968 Dec;199(3):637-84 PMID: 5710425
Time-resolved X-ray diffraction studies of the myosin layer-line reflections during muscle contraction.
J Mol Biol. 1982 Jul 15;158(4):637-84 PMID: 6981706
Changes in sarcomere length during isometric tension development in frog skeletal muscle.
J Physiol. 1972 Dec;227(1):1-17 PMID: 4539586
Dependence of energy transduction in intact skeletal muscles on the time in tension.
Biophys J. 1977 May;18(2):161-72 PMID: 140712
Proposed mechanism of force generation in striated muscle.
Nature. 1971 Oct 22;233(5321):533-8 PMID: 4939977
Contraction kinetics of striated muscle fibres following quick changes in load.
J Physiol. 1966 Jun;184(3):511-34 PMID: 5963731
Muscle structure and theories of contraction.
Prog Biophys Biophys Chem. 1957;7:255-318 PMID: 13485191
The relation between force and speed in muscular contraction.
J Physiol. 1939 Jun 14;96(1):45-64 PMID: 16995114
Muscle compliance and the longitudinal transmission of mechanical impulses.
J Gen Physiol. 1974 Dec;64(6):623-42 PMID: 4548435
Segmental length changes in stimulated frog sartorius muscle during dynamic mechanical responses.
Jpn J Physiol. 1982;32(5):817-30 PMID: 7154433
The compliance of contracting skeletal muscle.
J Physiol. 1974 Mar;237(3):477-93 PMID: 4207658
On the relationships between membrane potential, calcium transient and tension in single barnacle muscle fibres.
J Physiol. 1970 Jul;209(1):105-30 PMID: 5499037
Activation delays in frog twitch muscle fibres.
J Physiol. 1981;313:81-100 PMID: 7277238
A time-resolved X-ray diffraction study of muscle during twitch.
J Physiol. 1978 May;278:297-307 PMID: 307597
Local movement in stimulated frog sartorius muscle.
J Gen Physiol. 1981 Aug;78(2):151-70 PMID: 6974226
Force-velocity relation in normal and nitrate-treated frog single muscle fibres during rise of tension in an isometric tetanus.
J Physiol. 1978 Dec;285:257-73 PMID: 311382
Muscular contraction: kinetics of crossbridge attachment studied by high-frequency stiffness measurements.
Science. 1982 Jul 2;217(4554):70-2 PMID: 6979780
Calcium transients in isolated amphibian skeletal muscle fibres: detection with aequorin.
J Physiol. 1978 Apr;277:291-323 PMID: 306438

Article Info

Journal

Biophysical journal

Abbr.

Biophys J

ISSN

0006-3495

DOI

10.1016/S0006-3495(84)84163-6

Published

1984-02-00

Pages

389-97

Language

English

Region

United States

NLM ID

0370626

PMCID

PMC1434863

Subset

External Links

PubMed source DOI: 10.1016/S0006-3495(84)84163-6 PMC Full Text View journal details

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Stiffness, force, and sarcomere shortening during a twitch in frog semitendinosus muscle bundles.

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