Home LiteratureArticle Details
PMID: 6603620 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of rat hypothalamic corticotropin-releasing factor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 15 ·1983-08-00 ·Pages 4851-5

Rivier J, Spiess J, Vale W

Abstract

A polypeptide was purified from rat hypothalamic extracts on the basis of its high intrinsic activity to release corticotropin (ACTH) from cultured rat anterior pituitary cells and its immunoactivity in a radioimmunoassay directed against the NH2 terminus (residues 4-20) of ovine hypothalamic corticotropin-releasing factor (CRF). Based on Edman degradation, peptide mapping, and amino acid analysis, the primary structure of this rat CRF was established to be: H-Ser-Glu-Glu-Pro-Pro-Ile-Ser-Leu-Asp-Leu-Thr-Phe-His-Leu-Leu-Arg-Glu-Val-Leu-Glu-Met-Ala-Arg-Ala-Glu-Gln-Leu-Ala-Gln-Gln-Ala-His-Ser-Asn-Arg-Lys-Leu-Met-Glu-Ile-Ile-NH2. The hypophysiotropic potency of synthetic rat CRF did not deviate significantly from the potencies of the isolated native peptide or of synthetic ovine CRF. The close structural relationship between rat and ovine hypothalamic CRF is indicated by an 83% sequence homology.

MeSH Terms
Amino Acid Sequence Animals Carps Corticotropin-Releasing Hormone/isolation & purification Hypothalamus/analysis Peptide Fragments/analysis Radioimmunoassay Rats Sheep Species Specificity
Chemicals
Peptide Fragments Corticotropin-Releasing Hormone
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rivier J
Spiess J
Vale W
References (28)
28 references, click to expand
  1. Corticotropin releasing factor produces behavioural activation in rats.
    Nature. 1982 May 27;297(5864):331-3 PMID: 6978997
  2. Close relationship of mitochondria with intercellular junctions in the adrenaline cells of the mouse adrenal gland.
    Experientia. 1982 Feb 15;38(2):270-1 PMID: 7060714
  3. Corticotropin-releasing factor: actions on the sympathetic nervous system and metabolism.
    Endocrinology. 1982 Sep;111(3):928-31 PMID: 7049676
  4. Corticotropin releasing activity of the new CRF is potentiated several times by vasopressin.
    Nature. 1982 Sep 23;299(5881):355-7 PMID: 6287293
  5. Presence of corticotropin releasing factor-like immunoreactivity in hypophysial portal blood.
    Endocrinology. 1982 Oct;111(4):1418-20 PMID: 6288364
  6. Corticotropin releasing factor (CRF): immunoreactive neurones and fibers in rat hypothalamus.
    Regul Pept. 1982 Jun;4(1):43-8 PMID: 6750704
  7. Comparison of the biologic actions of corticotropin-releasing factor and sauvagine.
    Regul Pept. 1982 Jul;4(2):107-14 PMID: 6289384
  8. Complete amino acid sequence of urotensin I, a hypotensive and corticotropin-releasing neuropeptide from Catostomus.
    Science. 1982 Oct 8;218(4568):162-5 PMID: 6981844
  9. Corticotropin-releasing factor (CRF): central effects on mean arterial pressure and heart rate in rats.
    Endocrinology. 1982 Jun;110(6):2222-4 PMID: 6978810
  10. Inhibition of adrenocorticotropic hormone secretion in the rat by immunoneutralization of corticotropin-releasing factor.
    Science. 1982 Oct 22;218(4570):377-9 PMID: 6289439
  11. Growth hormone-releasing factor from a human pancreatic tumor that caused acromegaly.
    Science. 1982 Nov 5;218(4572):585-7 PMID: 6812220
  12. Amunine (ovine CRF), urotensin I and sauvagine, three structurally-related peptides, produce selective dilation of the mesenteric circulation.
    Pharmacology. 1982;25(2):116-20 PMID: 6982480
  13. Characterization of a growth hormone-releasing factor from a human pancreatic islet tumour.
    Nature. 1982 Nov 18;300(5889):276-8 PMID: 6292724
  14. Cloning and sequence analysis of cDNA for ovine corticotropin-releasing factor precursor.
    Nature. 1983 Feb 10;301(5900):537-40 PMID: 6600512
  15. Radioimmunoassay and chromatographic characterisation of CRF-41-like immunoreactivity in hypothalami from several species.
    Neuropeptides. 1982 Oct;3(1):45-52 PMID: 6984143
  16. High-performance liquid chromatographic purification of peptide hormones: ovine hypothalamic amunine (corticotropin releasing factor).
    Anal Biochem. 1982 Dec;127(2):258-66 PMID: 6984596
  17. Effects of synthetic human pancreatic (tumor) GH releasing factor and somatostatin, triiodothyronine and dexamethasone on GH secretion in vitro.
    Endocrinology. 1983 Apr;112(4):1553-5 PMID: 6131812
  18. Organization of ovine corticotropin-releasing factor immunoreactive cells and fibers in the rat brain: an immunohistochemical study.
    Neuroendocrinology. 1983;36(3):165-86 PMID: 6601247
  19. The release of corticotrophin by anterior pituitary tissue in vitro.
    Can J Biochem Physiol. 1955 May;33(3):408-15 PMID: 14364332
  20. Humoral hypothalamic control of anterior pituitary: a study with combined tissue cultures.
    Endocrinology. 1955 Nov;57(5):599-607 PMID: 13270723
  21. Culture of enzymatically dispersed pituitary cells: functional validation of a method.
    Endocrinology. 1972 Aug;91(2):562-72 PMID: 4630101
  22. Use of octadecasilyl-silica for the extraction and purification of peptides in biological samples. Application to the identification of circulating metabolites of corticotropin-(1-24)-tetracosapeptide and somatostatin in vivo.
    Biochem J. 1977 Oct 15;168(1):9-13 PMID: 202257
  23. Empirical predictions of protein conformation.
    Annu Rev Biochem. 1978;47:251-76 PMID: 354496
  24. Isolation and characterization of hypothalamic peptide hormones.
    Curr Top Mol Endocrinol. 1976;3:355-72 PMID: 28907
  25. Isolation and amino acid composition of sauvagine. An active polypeptide from methanol extracts of the skin of the South American frog Phyllomedusa sauvagei.
    Int J Pept Protein Res. 1980 Sep;16(3):191-9 PMID: 7461901
  26. Characterization of a 41-residue ovine hypothalamic peptide that stimulates secretion of corticotropin and beta-endorphin.
    Science. 1981 Sep 18;213(4514):1394-7 PMID: 6267699
  27. Primary structure of corticotropin-releasing factor from ovine hypothalamus.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6517-21 PMID: 6273874
  28. In vitro potentiation of the activity of synthetic ovine corticotropin-releasing factor by arginine vasopressin.
    Peptides. 1982 Mar-Apr;3(2):111-3 PMID: 6285317
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.15.4851
Published
1983-08-00
Pages
4851-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC384143
Subset
IM
Grants
NIADDK NIH HHS · AM20917 · United States
NIADDK NIH HHS · AM26741 · United States
NICHD NIH HHS · HD13527 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.15.4851 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2