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PMID: 6602864 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sodium flux ratio through the amiloride-sensitive entry pathway in frog skin.

The Journal of general physiology ·Vol. 81 ·No. 5 ·1983-05-00 ·Pages 667-85

Benos DJ, Hyde BA, Latorre R

Abstract

The sodium flux ratio of the amiloride-sensitive Na+ channel in the apical membrane of in vitro Rana catesbeiana skin has been evaluated at different sodium concentrations and membrane potentials in sulfate Ringer solution. Amiloride-sensitive unidirectional influxes and effluxes were determined as the difference between bidirectional 22Na and 24Na fluxes simultaneously measured in the absence and presence of 10(-4) M amiloride in the external bathing solution. Amiloride-sensitive Na+ effluxes were induced by incorporation of cation-selective ionophores (amphotericin B or nystatin) into the normally Na+-impermeable basolateral membrane. Apical membrane potentials (Va) were measured with intracellular microelectrodes. We conclude that since the flux ratio exponent, n', is very close to 1, sodium movement through this channel can be explained by a free-diffusion model in which ions move independently. This result, however, does not necessarily preclude the possibility that this transport channel may contain one or more ion binding sites.

MeSH Terms
Amiloride/pharmacology Amphotericin B/pharmacology Animals Nystatin/pharmacology Pyrazines/pharmacology Rana catesbeiana Skin/metabolism Skin Physiological Phenomena Sodium/metabolism
Chemicals
Pyrazines Nystatin Amiloride Amphotericin B Sodium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Benos D J
Hyde B A
Latorre R
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41 references, click to expand
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Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
0022-1295
DOI
10.1085/jgp.81.5.667
Published
1983-05-00
Pages
667-85
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2216559
Subset
IM
Grants
NIADDK NIH HHS · AM-25886 · United States
NIGMS NIH HHS · GM-25277 · United States
External Links
PubMed source DOI: 10.1085/jgp.81.5.667 PMC Full Text View journal details
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